Sampling Error in Histopathology Findings of Nonalcoholic Fatty Liver Disease: A Post Mortem Liver Histology Study.
Background: Many clinical trials and natural history studies on nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) rely heavily on liver histology to define their endpoints. There are many indications that the liver is not uniformly involved in NAFLI thus sampling error...
| Publicado en: | Archives of Iranian Medicine (AIM) Vol. 15; no. 7; pp. 418 - 422 |
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| Autores principales: | , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Academy of Medical Sciences of I.R. Iran
Jul2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=78358475&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 78358475 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10292977 5DHN jtl: Archives of Iranian Medicine (AIM) issn: 10292977 maglogo: N pubinfo: dt: Jul2012 vid: 15 iid: 7 pid: 42042 pub: Academy of Medical Sciences of I.R. Iran place: , <Blank> artinfo: ui: 78358475 78358475 108141129 78358475 ppf: 418 ppct: 4 formats: tig: atl: Sampling Error in Histopathology Findings of Nonalcoholic Fatty Liver Disease: A Post Mortem Liver Histology Study. aug: au: Merat, Shahin Sotoudehmanesh, Rasoul Nouraie, Mehdi Peikan-Heirati, Masoumeh Sepanlou, Sadaf G. Malekzadeh, Reza Sotoudeh, Masoud affil: Digestive Disease Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran sug: subj: Fatty Liver Physiopathology Histology Methods Human Data Analysis Software Postmortem Care Male Autopsy Female Child Adolescence Adult Middle Age Aged, 80 and Over Child: 6-12 years Adolescent: 13-18 years Adult: 19-44 years Middle Aged: 45-64 years Aged, 80 & over Male Female ab: Background: Many clinical trials and natural history studies on nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) rely heavily on liver histology to define their endpoints. There are many indications that the liver is not uniformly involved in NAFLI thus sampling error is a major concern. This study aims to evaluate the uniformity of various histologic features in livers affected with NAFLC Methods: Samples from a forensic autopsy series were studied and subjects with NAFLD identified. We took specimens from three different parts of each liver and recorded the degrees of steatosis, hepatocyte ballooning, lobular inflammation, portal inflammation, and fibrosis. NASH activity index (NAI) which is the sum of scores of histologic features was also calculated. The agreement between the 3 samples from each liver was studied. Results: There were 945 autopsies performed; 896 were suitable for histologic evaluation and 283 had NAFLD. Of these, 146 livers were available to our study from which 438 samples were taken. Fibrosis[intra-class correlation (ICC) = 0.87], lobular inflammation (kappa = 0.83 and portal inflammation (kappa = 0.83)were fairly uniformly distributed in the damaged liver. Steatosis was less uniform (kappa = 0.64), and hepatocyte ballooning was least uniformly distributed (kappa = 0.57). The ICC for NAI was 0.86, which indicated good agreement. Conclusions: The individual histologic features of NAFLD and NASH are not uniformly distributed in the liver. Hepatocyte ballooning is especially non-uniform. Such non-uniformity should be taken into account when interpreting results of studies that rely on paired biopsies. A summary score such as NAI is less affected by sampling error. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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