Up-regulated expression of HLA-DRB5 transcripts and high frequency of the HLA-DRB5*01:05 allele in scleroderma patients with interstitial lung disease.
Objective. Interstitial lung disease (ILD) is a serious complication of SSc. We aimed to identify markers associated with SSc-related ILD.Methods. RNA was prepared from the peripheral blood mononuclear cells of 14 SSc patients, divided into four different RNA pools according to the presence or absen...
| Publicado en: | Rheumatology Vol. 51; no. 10; pp. 1765 - 1775 |
|---|---|
| Autores principales: | , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Oct2012
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=80412964&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 80412964 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14620324 DN9 jtl: Rheumatology issn: 14620324 maglogo: N pubinfo: dt: Oct2012 vid: 51 iid: 10 pid: 622 pub: Oxford University Press / USA artinfo: ui: 80412964 104418866 104418866 10.1093/rheumatology/kes149 80412964 ppf: 1765 ppct: 10 formats: tig: atl: Up-regulated expression of HLA-DRB5 transcripts and high frequency of the HLA-DRB5*01:05 allele in scleroderma patients with interstitial lung disease. aug: au: Odani, Toshio Yasuda, Shinsuke Ota, Yuko Fujieda, Yuichiro Kon, Yujiro Horita, Tetsuya Kawaguchi, Yasushi Atsumi, Tatsuya Yamanaka, Hisashi Koike, Takao affil: Department of Medicine II, Hokkaido University Graduate School of Medicine, Sapporo and Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, Japan. sug: subj: Scleroderma, Systemic Complications Lung Diseases, Interstitial Familial and Genetic Risk Assessment Pulmonary Fibrosis Familial and Genetic Reverse Transcriptase Polymerase Chain Reaction Utilization Genotype Case Control Studies Japan Academic Medical Centers Male Female Adult Middle Age Equipment and Supplies Descriptive Statistics Autoantibodies Data Analysis Software Mann-Whitney U Test Chi Square Test Fisher's Exact Test Post Hoc Analysis Multivariate Analysis Logistic Regression Human Odds Ratio Confidence Intervals Scleroderma, Systemic Symptoms Adult: 19-44 years Middle Aged: 45-64 years Male Female ab: Objective. Interstitial lung disease (ILD) is a serious complication of SSc. We aimed to identify markers associated with SSc-related ILD.Methods. RNA was prepared from the peripheral blood mononuclear cells of 14 SSc patients, divided into four different RNA pools according to the presence or absence of ILD and to the treatment, and subjected to microarray analysis. Real-time quantitative PCR was used to confirm the microarray results in 43 SSc patients, 42 autoimmune controls and 10 healthy controls. Genomic DNA samples were collected from 149 patients with SSc (70 in Hokkaido and 79 in Tokyo) who underwent a high-resolution CT for the evaluation of ILD and from 230 healthy controls. Genotyping was performed using sequence-specific primers.Results. The microarray analysis revealed HLA-DRB5 to be the only gene commonly up-regulated in patients with ILD compared with those without ILD in both comparison groups. High expression levels of HLA-DRB5 in SSc patients with ILD were confirmed by real-time quantitative PCR. The prevalence of HLA-DRB5 gene carriers increased in the SSc patients with ILD relative to those without ILD or to healthy controls in both cohorts. Among the four detected alleles, the HLA-DRB5*01:05 allele was significantly more frequent in SSc patients with ILD than in SSc patients without ILD or in healthy controls. These associations were confirmed in the second cohort.Conclusion. HLA-DRB5 was highly expressed in PBMCs from patients with SSc-related ILD. The HLA-DRB5*01:05 allele is a risk factor for ILD in patients with SSc. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|