The Effect of DNA Vaccine Induced IgY Against C-Urease in Mice Infected with Helicobacter Pylori.
Background and Objectives: Helicobacter pylori is a gram negative, spiral bacterium that colonizes and infects human gastric mucosal. Infection by these bacteria causes chronic inflammation of stomach leading to stomach and duodenum ulcers. Aim of the present research is to study the effect of IgY p...
| Publicado en: | Medical Journal of Tabriz University of Medical Sciences & Health Services Vol. 34; no. 5; pp. 21 - 22 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Tabriz University of Medical Sciences
Dec2012-Jan2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=85364481&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 85364481 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16085671 EUW8 jtl: Medical Journal of Tabriz University of Medical Sciences & Health Services issn: 16085671 maglogo: N pubinfo: dt: Dec2012-Jan2013 vid: 34 iid: 5 pid: 69573 pub: Tabriz University of Medical Sciences artinfo: ui: 85364481 85364481 ppf: 21 ppct: 1 formats: fmt: @attributes: type: P tig: atl: The Effect of DNA Vaccine Induced IgY Against C-Urease in Mice Infected with Helicobacter Pylori. aug: au: Malekshahi, Ziba Vaise Gargari, Seyedlatif Mousavi Salmanian, Ali Hatef Ebrahimizadeh, Walead Javid, Shaghayegh Amiri affil: Department of Biology, School of Basic Science, Shahed University, Tehran, Iran sug: ab: Background and Objectives: Helicobacter pylori is a gram negative, spiral bacterium that colonizes and infects human gastric mucosal. Infection by these bacteria causes chronic inflammation of stomach leading to stomach and duodenum ulcers. Aim of the present research is to study the effect of IgY purified from eggs yolk of immunized with DNA vaccine of Curease, a subunit of urease enzyme, in treatment of mice infected with H. pylori. Materials and Methods: Inoculums of 109 bacteria in 400μl Brocella broth medium were gavaged into C57BL/6j mice in an alternate day method in a six day period. 400 μl of Brocella broth medium was given to the control mice. Eight weeks after the last inoculation, blood was taken from the mice, and infection rate was analyzed using indirect ELISA method. Severity of inflammation was analyzed by pathological tests. Infected mice were orally treated for 28 days with 60 mg of IgY isolated from hens immunized with DNA vaccine. Results: ELISA results showed establishment of infection in 53% of the infected mice. Pathological results, confirmed the inflammation in the infected subjects. Significant difference in antibody titer of serum was observed between infected mice treated with IgY and the positive control group. Conclusion: This study showed that C-urease induced IgY is successful in inhibition of HPylori infection in mice model. pubtype: Academic Journal doctype: Journal Article ougenre: Unknown language: English refInfo: holdings: @attributes: islocal: N |
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