Enzyme Molecules as Nanomotors.
Using fluorescence correlation spectroscopy, we show that the diffusive movements of catalase enzyme molecules increase in the presence of the substrate, hydrogen peroxide, in a concentration-dependent manner. Employing a microfluidic device to generate a substrate concentration gradient, we show th...
| Publicado en: | Journal of the American Chemical Society Vol. 135; no. 4; pp. 1406 - 1415 |
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| Autores principales: | , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
1/30/2013
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=85660124&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 85660124 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 1/30/2013 vid: 135 iid: 4 pid: 997 pub: American Chemical Society artinfo: ui: 85660124 10.1021/ja3091615 ppf: 1406 ppct: 9 formats: tig: atl: Enzyme Molecules as Nanomotors. aug: au: Sengupta, Samudra Dey, Krishna K. Muddana, Hari S. Tabouillot, Tristan Ibele, Michael E. Butler, Peter J. Sen, Ayusman affil: Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, United States Department of Bioengineering, Pennsylvania State University, University Park, Pennsylvania 16802, United States su: Enzymes Nanostructures Fluorescence spectroscopy Catalase Substrates (Materials science) Microfluidics Urease Chemotaxis sug: subj: Enzymes Nanostructures Fluorescence spectroscopy Catalase Substrates (Materials science) Microfluidics Urease Chemotaxis ab: Using fluorescence correlation spectroscopy, we show that the diffusive movements of catalase enzyme molecules increase in the presence of the substrate, hydrogen peroxide, in a concentration-dependent manner. Employing a microfluidic device to generate a substrate concentration gradient, we show that both catalase and urease enzyme molecules spread toward areas of higher substrate concentration, a form of chemotaxis at the molecular scale. Using glucose oxidase and glucose to generate a hydrogen peroxide gradient, we induce the migration of catalase toward glucose oxidase, thereby showing that chemically interconnected enzymes can be drawn together. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2013 holdings: @attributes: islocal: N |
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