Leukocyte transcript alterations in West-African girls following a booster vaccination with diphtheria-tetanus-pertussis vaccine.
Background: Observational studies from low-income countries have shown that the vaccination against diphtheria, tetanus and pertussis (DTP) is associated with excess female mortality due to infectious diseases.Methods: To investigate possible changes in gene expression after DTP vaccination, we iden...
| Publicado en: | Scandinavian Journal of Clinical & Laboratory Investigation Vol. 73; no. 4; pp. 349 - 355 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | research randomized controlled trial Journal Article |
| Publicado: |
Taylor & Francis Ltd
Jun2013
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=88104905&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 88104905 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00365513 BD5 jtl: Scandinavian Journal of Clinical & Laboratory Investigation issn: 00365513 maglogo: Y pubinfo: dt: Jun2013 vid: 73 iid: 4 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 88104905 88104905 NLM23668887 88104905 10.3109/00365513.2013.783229 NLM23668887 88104905 ppf: 349 ppct: 6 formats: tig: atl: Leukocyte transcript alterations in West-African girls following a booster vaccination with diphtheria-tetanus-pertussis vaccine. aug: au: Ørntoft, Nikolaj W. Thorsen, Kasper Benn, Christine S. Lemvik, Grethe Nanque, Joao R. Aaby, Peter Østergaard, Lars Agergaard, Jane affil: Department of Medicine V (Hepatology and Gastroenterology), Aarhus University Hospital, Aarhus, Denmark sug: subj: Diphtheria-Tetanus-Pertussis Vaccine Adverse Effects Immunization, Secondary Adverse Effects Leukocytes Immunology Gene Expression Gene Expression Profiling Pilot Studies Molecular Structure Infant Africa, Western Interferons Human Interferons Blood Female Validation Studies Comparative Studies Evaluation Research Multicenter Studies Randomized Controlled Trials Infant: 1-23 months Female ab: Background: Observational studies from low-income countries have shown that the vaccination against diphtheria, tetanus and pertussis (DTP) is associated with excess female mortality due to infectious diseases.Methods: To investigate possible changes in gene expression after DTP vaccination, we identified a group of nine comparable West African girls, from a biobank of 356 children, who were due to receive DTP booster vaccine at age 18 months. As a pilot experiment we extracted RNA from blood samples before, and 6 weeks after, vaccination to analyze the coding transcriptome in leukocytes using expression microarrays, and ended up with information from eight girls. The data was further analyzed using dedicated array pathway and network software. We aimed to study whether DTP vaccination introduced a systematic alteration in the immune system in girls.Results: We found very few transcripts to alter systematically. Those that did mainly belonged to the Interferon (IFN) signalling pathway. We scrutinized this pathway as well as the Interleukin (IL) pathways. Two out of eight showed a down-regulated IFN pathway and two showed an up-regulated IFN pathway. The two with down-regulated IFN pathway had also down-regulated IL-6 pathway. In the study of networks, two of the girls stood out as not having the inflammatory response as top altered network.Conclusion: The transcriptome changes following DTP booster vaccination were subtle, but although the material was small, it was possible to identify sub groups that deviate from each other, mainly in the IFN response. pubtype: Academic Journal doctype: research randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|