In vitro antifungal susceptibility pattern and ergosterol content in clinical yeast strains.
Background: Absence or severe reduction in the amount of ergosterol in the fungal membrane and its replacement with other sterols have been described as potential antifungal resistance mechanisms in fungi. Aims and methods: The ergosterol content in a collection of 51 clinical yeast isolates, includ...
| Publicado en: | Revista Iberoamericana de Micologia Vol. 28; no. 1; pp. 100 - 104 |
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| Autores principales: | , , , |
| Formato: | Artículo |
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Elsevier B.V.
2011
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=89172153&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 89172153 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 11301406 PVP jtl: Revista Iberoamericana de Micologia issn: 11301406 maglogo: N pubinfo: dt: 2011 vid: 28 iid: 1 pid: 467 pub: Elsevier B.V. artinfo: ui: 89172153 10.1016/j.riam.2010.12.003 ppf: 100 ppct: 4 formats: fmt: @attributes: type: P size: 107KB tig: atl: In vitro antifungal susceptibility pattern and ergosterol content in clinical yeast strains. aug: au: Gomez-Lopez, Alicia Buitrago, María Jose Rodriguez-Tudela, Juan Luis. Cuenca-Estrella, Manuel affil: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, España su: Antifungal agents Microbial sensitivity tests Ergosterol Medical microbiology Yeast Chromatographic analysis Microbiology sug: subj: Antifungal agents Microbial sensitivity tests Ergosterol Medical microbiology Yeast Chromatographic analysis Microbiology keyword: HPLC-UV Levaduras Mecanismos de resistencia Resistance mechanisms Yeasts Levaduras Mecanismos de resistencia ab: Background: Absence or severe reduction in the amount of ergosterol in the fungal membrane and its replacement with other sterols have been described as potential antifungal resistance mechanisms in fungi. Aims and methods: The ergosterol content in a collection of 51 clinical yeast isolates, including susceptible and resistant strains to amphotericin B and azoles, was estimated by a simple chromatographic method (HPLC-UV). Results: A high content of ergosterol was detected for several strains of Candida glabrata, Candida tropi- calis or Pichia membranifaciens. In contrast, strains of Cryptococcus neoformans and Dipodascus capitatus had the lowest ergosterol concentrations. No significant correlation was observed between antifungal susceptibility patterns and ergosterol content. Conclusions: We conclude from this study that ergosterol content on yeasts may not be associated with specific resistant patterns. Antecedentes: La ausencia o disminución en la cantidad de ergosterol, así como su sustitución por otros esteroles en la membrana, se ha considerado como un posible mecanismo de resistencia de la célula fúngica. Objetivos y métodos: En este trabajo hemos evaluado la cantidad de ergosterol de una colección de 51 aislamientos clínicos de levaduras, incluyendo cepas sensibles y resistentes a antifúngicos, mediante un sencillo método cromatográfico (HPLC-UV). Resultados: Algunas cepas de Candida glabrata, Candida tropicalis y Pichia membranifaciens mostraron mayor contenido en ergosterol que el resto, mientras que las de Cryptococcus neoformans y Dipodascus capitatus presentaron el contenido más bajo. No se observó ninguna relación con suficiente potencia estadística entre el patrón de sensibilidad in vitro y el contenido de ergosterol. Conclusiones: Podemos concluir de este estudio que el contenido en ergosterol no se relaciona sistemáticamente con un patrón de resistencia definido. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Iberoamericana de Micologia is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Iberoamericana de Micologia holder: Elsevier B.V. dt: @attributes: year: 2011 holdings: @attributes: islocal: N |
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