Glycosylation status of serum in inflammatory arthritis in response to anti-TNF treatment.
Objective. Glycosylation is the most common post-translational modification and is altered in disease. The typical glycosylation change in patients with inflammatory arthritis (IA) is a decrease in galactosylation levels on IgG. The aim of this study is to evaluate the effect of anti-TNF therapy on...
| Publicado en: | Rheumatology Vol. 52; no. 9; pp. 1572 - 1583 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Sep2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=89734414&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 89734414 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14620324 DN9 jtl: Rheumatology issn: 14620324 maglogo: N pubinfo: dt: Sep2013 vid: 52 iid: 9 pid: 622 pub: Oxford University Press / USA artinfo: ui: 89734414 104210225 104210225 10.1093/rheumatology/ket189 89734414 ppf: 1572 ppct: 11 formats: tig: atl: Glycosylation status of serum in inflammatory arthritis in response to anti-TNF treatment. aug: au: Collins, Emily S. Galligan, Marie C. Saldova, Radka Adamczyk, Barbara Abrahams, Jodie L. Campbell, Matthew P. Ng, Chin-Teck Veale, Douglas J. Murphy, Thomas B. Rudd, Pauline M. FitzGerald, Oliver affil: Department of Rheumatology, Dublin Academic Medical Centre, St Vincent's University Hospital, Elm Park and The Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland, UCD School of Mathematical Sciences, University College Dublin, Dublin, Ireland, NIBRT Dublin-Oxford Glycobiology Laboratory, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Dublin 4, Ireland and Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. sug: subj: Arthritis Drug Therapy Tumor Necrosis Factor Antagonists and Inhibitors Glycosylation Human Arthritis, Rheumatoid Drug Therapy Arthritis, Psoriatic Drug Therapy C-Reactive Protein Blood Academic Medical Centers Ireland Mann-Whitney U Test Logistic Regression Funding Source ab: Objective. Glycosylation is the most common post-translational modification and is altered in disease. The typical glycosylation change in patients with inflammatory arthritis (IA) is a decrease in galactosylation levels on IgG. The aim of this study is to evaluate the effect of anti-TNF therapy on whole serum glycosylation from IA patients and determine whether these alterations in the glycome change upon treatment of the disease.Methods. Serum samples were collected from 54 IA patients before treatment and at 1 and 12 months after commencing anti-TNF therapy. N-linked glycans from whole serum samples were analysed using a high-throughput hydrophilic interaction liquid chromatography-based method.Results. Glycosylation on the serum proteins of IA patients changed significantly with anti-TNF treatment. We observed an increase in galactosylated glycans from IgG, also an increase in core-fucosylated biantennary galactosylated glycans and a decrease in sialylated triantennary glycans with and without outer arm fucose. This increase in galactosylated IgG glycans suggests a reversing of the N-glycome towards normal healthy profiles. These changes are strongly correlated with decreasing CRP, suggesting a link between glycosylation changes and decreases in inflammatory processes.Conclusion. Glycosylation changes in the serum of IA patients on anti-TNF therapy are strongly associated with a decrease in inflammatory processes and reflect the effect of anti-TNF on the immune system. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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