Association Dynamics and Linear and Nonlinear Optical Properties of an N-Acetylaladanamide Probe in a POPC Membrane.
Along with the growing evidence that relates membrane abnormalities to various diseases, biological membranes have been acknowledged as targets for therapy. Any such abnormality in the membrane structure alters the membrane potential which in principle can be captured by measuring properties of spec...
| Publicado en: | Journal of the American Chemical Society Vol. 135; no. 36; pp. 13590 - 13598 |
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| Autores principales: | , , , , , |
| Formato: | Artículo |
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American Chemical Society
9/11/2013
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=90490928&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 90490928 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 9/11/2013 vid: 135 iid: 36 pid: 997 pub: American Chemical Society artinfo: ui: 90490928 10.1021/ja407326n ppf: 13590 ppct: 8 formats: tig: atl: Association Dynamics and Linear and Nonlinear Optical Properties of an N-Acetylaladanamide Probe in a POPC Membrane. aug: au: Arul Murugan, N. Apostolov, Rossen Rinkevicius, Zilvinas Kongsted, Jacob Lindahl, Erik Ågren, Hans affil: Division of Theoretical Chemistry and Biology, School of Biotechnology, Royal Institute of Technology, SE-10691 Stockholm, Sweden PDC Center for High Performance Computing, School of Computer Science and Communication, Royal Institute of Technology, SE-10691 Stockholm, Sweden Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, SE-171 21 Solna, Sweden Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark Theoretical and Computational Biophysics, Department of Theoretical Physics, Royal Institute of Technology, Stockholm, Sweden su: Second harmonic generation Molecular probes Biological membranes Nonlinear optics Membrane potential Microscopy sug: subj: Second harmonic generation Molecular probes Biological membranes Nonlinear optics Membrane potential Microscopy ab: Along with the growing evidence that relates membrane abnormalities to various diseases, biological membranes have been acknowledged as targets for therapy. Any such abnormality in the membrane structure alters the membrane potential which in principle can be captured by measuring properties of specific optical probes. There exists by now many molecular probes with absorption and fluorescence properties that are sensitive to local membrane structure and to the membrane potential. To suggest new high-performance optical probes for membrane-potential imaging it is important to understand in detail the membrane-induced structural changes in the probe, the membrane association dynamics of the probe, and its membrane-specific optical properties. To contribute to this effort, we here study an optical probe, N-acetylaladanamide (NAAA), in the presence of a POPC lipid bilayer using a multiscale integrated approach to assess the probe structure, dynamics, and optical properties in its membrane-bound status and in water solvent. We find that the probe eventually assimilates into the membrane with a specific orientation where the hydrophobic part of the probe is buried inside the lipid bilayer, while the hydrophilic part is exposed to the water solvent. The computed absorption maximum is red-shifted when compared to the gas phase. The computations of the two-photon absorption and second harmonic generation cross sections of the NAAA probe in its membrane-bound state which is of its first kind in the literature suggest that this probe can be used for imaging the membrane potential using nonlinear optical microscopy. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2013 holdings: @attributes: islocal: N |
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