Activatable Oligomerizable Imaging Agents for Photoacoustic Imaging of Furin-Like Activity in Living Subjects.
Photoacoustic (PA) imaging is continuing to be applied for physiological imaging and more recently for molecular imaging of living subjects. Owing to its high spatial resolution in deep tissues, PA imaging holds great potential for biomedical applications and molecular diagnostics. There is however...
| Publicado en: | Journal of the American Chemical Society Vol. 135; no. 30; pp. 11015 - 11023 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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American Chemical Society
7/31/2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=90529987&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 90529987 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 7/31/2013 vid: 135 iid: 30 pid: 997 pub: American Chemical Society artinfo: ui: 90529987 10.1021/ja4010078 ppf: 11015 ppct: 8 formats: tig: atl: Activatable Oligomerizable Imaging Agents for Photoacoustic Imaging of Furin-Like Activity in Living Subjects. aug: au: Dragulescu-Andrasi, Anca Kothapalli, Sri-Rajasekhar Tikhomirov, Grigory A. Jianghong Rao Gambhir, Sanjiv S. affil: Molecular Imaging Program, Stanford and Bio-X Program, Stanford University School of Medicine, Stanford, California 94305, United States Department of Radiology, Stanford University School of Medicine, Stanford, California 94305, United States Department of Bioengineering, Stanford University School of Medicine, Stanford, California 94305, United States Department of Materials Science and Engineering, Stanford University School of Medicine, Stanford, California 94305, United States su: Acoustic imaging Furin protein Molecular probes Proteolytic enzymes Oligomerization Enzyme activation sug: subj: Acoustic imaging Furin protein Molecular probes Proteolytic enzymes Oligomerization Enzyme activation ab: Photoacoustic (PA) imaging is continuing to be applied for physiological imaging and more recently for molecular imaging of living subjects. Owing to its high spatial resolution in deep tissues, PA imaging holds great potential for biomedical applications and molecular diagnostics. There is however a lack of probes for targeted PA imaging, especially in the area of enzyme-activatable probes. Here we introduce a molecular probe, which upon proteolytic processing is retained at the site of enzyme activity and provides PA contrast. The probe oligomerizes via a condensation reaction and accumulates in cells and tumors that express the protease. We demonstrate that this probe reports furin and furin-like activity in cells and tumor models by generating a significantly higher PA signal relative to furin-deficient and nontarget controls. This probe could report enzyme activity in living subjects at depths significantly greater than fluorescence imaging probes and has potential for molecular imaging in deep tumors. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2013 holdings: @attributes: islocal: N |
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