Bioanalysis for Biocatalysis: Multiplexed Capillary Electrophoresis-Mass Spectrometry Assay for Aminotransferase Substrate Discovery and Specificity Profiling.

In this work, we introduce an entirely automated enzyme assay based on capillary electrophoresis coupled to electrospray ionization mass spectrometry termed MINISEP-MS for multiple interfluent nanoinjections-incubation-separation-enzyme profiling using mass spectrometry. MINISEP-MS requires only nan...

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Publicado en:	Journal of the American Chemical Society Vol. 135; no. 37; pp. 13728 - 13737
Autores principales:	Mironov, Gleb G., St-Jacques, Antony D., Mungham, Alexander, Eason, Matthew G., Chica, Roberto A., Berezovski, Maxim V.
Formato:	Artículo
Publicado:	American Chemical Society 9/18/2013
Materias:	Catalysis Capillary electrophoresis Mass spectrometry Aminotransferases Microreactors Biochemical substrates Enzymes Amino acids
Acceso en línea:	Ver este registro en EBSCOhost