The Assessment of Proliferation Rate of Dental Pulp Stem Cells and Stem Cell from Human Exfoliated Deciduous Teeth by Using Two Different Scaffold.
Introduction: Dental pulp stem cells (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) were identified to be highly proliferative and clonogenic cells. Objectives: The objective was to assess the proliferation rate of DPSC and SHED in presence of two different scaffolds, synthetic g...
| Publicado en: | International Medical Journal Vol. 20; no. 5; pp. 593 - 597 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Japan International Cultural Exchange Foundation
Oct2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=92574114&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 92574114 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13412051 23J1 jtl: International Medical Journal issn: 13412051 maglogo: N pubinfo: dt: Oct2013 vid: 20 iid: 5 pid: 29386 pub: Japan International Cultural Exchange Foundation artinfo: ui: 92574114 92574114 107939897 92574114 ppf: 593 ppct: 4 formats: fmt: @attributes: type: P tig: atl: The Assessment of Proliferation Rate of Dental Pulp Stem Cells and Stem Cell from Human Exfoliated Deciduous Teeth by Using Two Different Scaffold. aug: au: Nawi, Nurul Saadah Binti Mat Ariffín, Zaihan Alam, Mohammad Khursheed Noor, Siti Noor Fazliah Mohd Hassan, Akram affil: School of Dental Sciences, Universiti Sains Malaysia Kubang Kerian, 16150 Kelantan, Malaysia sug: subj: Tooth, Deciduous Dental Pulp Stem Cells Cell Physiology Human Enzyme-Linked Immunosorbent Assay Time Factors Cell Culture Techniques Descriptive Statistics Chi Square Test Kruskal-Wallis Test Funding Source ab: Introduction: Dental pulp stem cells (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) were identified to be highly proliferative and clonogenic cells. Objectives: The objective was to assess the proliferation rate of DPSC and SHED in presence of two different scaffolds, synthetic granular hjdroxj apetite bone graft (Granumas®) and coral scaffolds. Materials and Methods: All teeth were extracted and isolation of the stem cells from dental pulp was done following standard protocol. The stem cells were cultured with a Modified Eagle's Medium (GIBCO/BRL) supplemented with 10% fetal Bovine serum (Equitec-Bio, Kerrville, TX, 100 fan L- glutamine (Wako Pure Chemicals, Osaka), 100 unit/ml penicillin, and 100 mg/ml streptomycin (Biofluids, Rokville, MD). The cells proliferation rate of the DPSC and SHED with scaffolds (Granumas® and coral scaffolds) and without scaffolds was assessed using Alamar Blue (Biosource Internatioal, Inc, USA) and the absorbance of the media was measured spectrophotometrically using ELISA plate reader (Tecan, DKSH) at a wavelength of 570 nm and 600 nm at 0 minute, 60 minutes, 120 minutes, 180 minutes, 240 minutes at day 0, day 4 and day 7. Results and Conclusion: The proliferation rate of DPSC is higher compared to SHED. Cancellous coral scaffold is more suitable in promoting stem cells differentiation and proliferation compared to synthetic hydroxyapetite granular bone graft. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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