Communication and Computation by Bacteria Compartmentalized within Microemulsion Droplets.
Amphiphilic inducer molecules such as Nacyl- L-homoserine lactones (AHLs) or isopropyl-β-D-thiogalactopyranoside (IPTG) can be utilized for the implementation of an artificial communication system between groups of E. coli bacteria encapsulated within water-in-oil microemulsion droplets. Using spati...
| Publicado en: | Journal of the American Chemical Society Vol. 136; no. 1; pp. 72 - 76 |
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| Autores principales: | , , , , , |
| Formato: | Artículo |
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American Chemical Society
1/8/2014
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=93882942&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 93882942 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 1/8/2014 vid: 136 iid: 1 pid: 997 pub: American Chemical Society artinfo: ui: 93882942 10.1021/ja411132w ppf: 72 ppct: 4 formats: tig: atl: Communication and Computation by Bacteria Compartmentalized within Microemulsion Droplets. aug: au: Weitz, Maximilian Mückl, Andrea Kapsner, Korbinian Berg, Ronja Meyer, Andrea Simmel, Friedrich C. affil: Physics Department E14 and ZNN/WSI, Technische Universität München, Am Coulombwall 4a, D-85748 Garching, Germany su: Microemulsions Amphiphiles Lactones Gene regulatory networks Microdroplets Gene expression Escherichia coli Diffusion coefficients sug: subj: Microemulsions Amphiphiles Lactones Gene regulatory networks Microdroplets Gene expression Escherichia coli Diffusion coefficients ab: Amphiphilic inducer molecules such as Nacyl- L-homoserine lactones (AHLs) or isopropyl-β-D-thiogalactopyranoside (IPTG) can be utilized for the implementation of an artificial communication system between groups of E. coli bacteria encapsulated within water-in-oil microemulsion droplets. Using spatially extended arrays of microdroplets, we study the diffusion of both AHL and IPTG from inducer-filled reservoirs into bacteria-containing droplets, and also from droplets with AHL producing sender bacteria into neighboring droplets containing receiver cells. Computational modeling of gene expression dynamics within the droplets suggests a strongly reduced effective diffusion coefficient of the inducers, which markedly affects the spatial communication pattern in the neighborhood of the senders. Engineered bacteria that integrate AHL and IPTG signals with a synthetic AND gate gene circuit are shown to respond only in the presence of both types of sender droplets, which demonstrates the potential of the system for genetically programmed pattern formation and distributed computing. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2014 holdings: @attributes: islocal: N |
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