Antidepressant effects of crocin and its effects on transcript and protein levels of CREB, BDNF, and VGF in rat hippocampus.
Antidepressants have been shown to affect levels of brain-derived neurotrophic factor (BDNF) and VGF (non-acronymic) whose transcriptions are dependent on cAMP response element binding protein (CREB) in long term treatment. The aim of this study was to verify the subacute antidepressant effects of c...
| Publicado en: | DARU: Journal of Pharmaceutical Sciences Vol. 22; pp. 1 - 18 |
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| Autores principales: | , , , , , |
| Formato: | research randomized controlled trial Journal Article |
| Publicado: |
Springer Nature
2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=94587820&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 94587820 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15608115 8WS jtl: DARU: Journal of Pharmaceutical Sciences issn: 15608115 maglogo: N pubinfo: dt: 2014 vid: 22 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 94587820 94587820 94587820 10.1186/2008-2231-22-16 94587820 ppf: 1 ppct: 17 formats: tig: atl: Antidepressant effects of crocin and its effects on transcript and protein levels of CREB, BDNF, and VGF in rat hippocampus. aug: au: Hassani, Faezeh Vahdati Naseri, Vahideh Razavi, Bibi Marjan Mehri, Soghra Abnous, Khalil Hosseinzadeh, Hossein affil: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran sug: subj: Antidepressive Agents Hippocampus Carotenoids Administration and Dosage Carotenoids Classification Plant Extracts Animal Studies Rats Polymerase Chain Reaction Blotting, Western Randomized Controlled Trials Funding Source ab: Antidepressants have been shown to affect levels of brain-derived neurotrophic factor (BDNF) and VGF (non-acronymic) whose transcriptions are dependent on cAMP response element binding protein (CREB) in long term treatment. The aim of this study was to verify the subacute antidepressant effects of crocin, an active constituent of saffron (Crocus sativus L.), and its effects on CREB, BDNF, and VGF proteins, transcript levels and amount of active, phosphorylated CREB (P-CREB) protein in rat hippocampus.Methods Crocin (12.5, 25, and 50 mg/kg), imipramine (10 mg/kg; positive control) and saline (1 mL/kg; neutral control) were administered intraperitoneally (IP) to male Wistar rats for 21 days. The antidepressant effects were studied using the forced swimming test (FST) on day 21 after injection. Protein expression and transcript levels of genes in the rat hippocampus were evaluated using western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR), respectively. Results Crocin significantly reduced the immobility time in the FST. Western blot analysis showed that 25 and 50 mg/kg of crocin increased the levels of CREB and BDNF significantly and dose dependently. All doses of crocin increased the VGF levels in a dose-dependent manner. Levels of p-CREB increased significantly by 50 mg/kg dose of crocin. Only 12.5 mg/kg crocin could significantly increase the transcript levels of BDNF. No changes in CREB and VGF transcript levels were observed in all groups. Conclusions These results suggest that crocin has antidepressant-like action by increasing CREB, BDNF and VGF levels in hippocampus. pubtype: Academic Journal doctype: research randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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