VEGF and bFGF Expression and Histological Characteristics of the Bone-Tendon Junction during Acute Injury Healing.

Bone-tendon junction (BTJ) injuries are common and may be caused by acute trauma and delayed healing during exercise or work. To understand the nature of the healing process of BTJ injuries would help to prevent injuries and improve treatment. Thirty-three mature female rabbit hindlimbs were assigne...

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Publicado en:Journal of Sports Science & Medicine Vol. 13; no. 1; pp. 15 - 22
Autores principales: Lin Wang, Weiwei Gao, Kaiyu Xiong, Kuan Hu, Xincun Liu, Hui He
Formato: diagnostic images pictorial research tables/charts Journal Article
Publicado: Hakan Gur, Journal of Sports Science & Medicine 2014
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Hakan Gur, Journal of Sports Science & Medicine
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        atl: VEGF and bFGF Expression and Histological Characteristics of the Bone-Tendon Junction during Acute Injury Healing.
      aug:
        au:
          Lin Wang
          Weiwei Gao
          Kaiyu Xiong
          Kuan Hu
          Xincun Liu
          Hui He
        affil: Section of Sports Medicine, Beijing Sport University, Beijing, China
      sug:
        subj:
          Bone and Bones Injuries
          Tendon Injuries
          Wound Healing
          China
          Funding Source
          Animal Studies
          In Vivo Studies
          Rabbits
          Sports Science
          Histological Techniques
          Fibroblasts
          Growth Substances Metabolism
          Vascular Endothelial Growth Factors Metabolism
          Descriptive Statistics
          Immunohistochemistry
          One-Way Analysis of Variance
          Data Analysis Software
      ab: Bone-tendon junction (BTJ) injuries are common and may be caused by acute trauma and delayed healing during exercise or work. To understand the nature of the healing process of BTJ injuries would help to prevent injuries and improve treatment. Thirty-three mature female rabbit hindlimbs were assigned to normal control (CON, n = 7) and injury groups (n = 26). The acute injury was established by administering one 7 plumblossom needle puncture. Specimens were harvested post injury at 1, 2, 4, and 8 weeks (ND1W, n = 6; ND2W, n = 6; ND4W, n = 7; and ND8W, n = 7). The injury existed in all of the injury groups. Compared with the CON group, all of the animals in the injury group showed poor cell profiles, an unclear or undetectable tide mark, a proteoglycan area and profile changes; the BTJ cell density diminished significantly in the ND1W (p < 0.01), ND2W (p < 0.05), ND4W (p < 0.01), and ND8W groups (p < 0.01); the fibrocartilage zone thickness in all injury groups was significantly thicker than in the CON group (p < 0.05), but no significant difference was found among the injury groups (p>0.05). The basic fibroblast growth factor (bFGF) expression in the CON group was significantly less than in the ND1W group (p<0.01), but no significant difference was found when compared with the ND2W, ND4W, and ND8W groups. The bFGF expression in the ND1W group was higher than that of the ND4W (p < 0.05) and ND8W groups (p < 0.01). The vascular endothelial growth factor (VEGF) levels were not significantly different among the groups (p > 0.05). The bFGF and VEGF expression levels indicated that the healing process stopped at 8 weeks post injury or was not activated, although the injury had not healed by histological examination. A repeatable animal model of BTJ acute injury was established in this study, and the results described the BTJ acute injury healing difficult concerned with the repairing stop.
      pubtype: Academic Journal
      doctype:
        diagnostic images
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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