Immunodetection of Helicobacter pylori-specific Proteins in Oral and Gastric Candida Yeasts.
Background: Human gastric epithelium and immunocytes have been recognized as the sole specialized eukaryotic cells that host Helicobacter pylori (H. pylori). The aim of this study was to provide further evidence for our previous proposal regarding the occurrence of H.pylori inside the yeast vacuole,...
| Publicado en: | Archives of Iranian Medicine (AIM) Vol. 16; no. 11; pp. 624 - 631 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Academy of Medical Sciences of I.R. Iran
Nov2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=95438112&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 95438112 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10292977 5DHN jtl: Archives of Iranian Medicine (AIM) issn: 10292977 maglogo: N pubinfo: dt: Nov2013 vid: 16 iid: 11 pid: 42042 pub: Academy of Medical Sciences of I.R. Iran place: , <Blank> artinfo: ui: 95438112 104066036 104066036 95438112 ppf: 624 ppct: 7 formats: tig: atl: Immunodetection of Helicobacter pylori-specific Proteins in Oral and Gastric Candida Yeasts. aug: au: Saniee, Parastoo Siavoshi, Farideh Broujeni, Gholamreza Nikbakht Khormali, Mahmood Sarrafnejad, Abdolfatah Malekzadeh, Reza affil: Department of Microbiology, School of Biology, University College of Sciences, University of Tehran, Tehran, Iran sug: subj: Helicobacter Pylori Analysis Immunologic Tests Methods Candida Proteins Analysis Microscopy Polymerase Chain Reaction Blotting, Western Sulfhydryl Reagents Gastrointestinal System Microbiology Electrophoresis Funding Source ab: Background: Human gastric epithelium and immunocytes have been recognized as the sole specialized eukaryotic cells that host Helicobacter pylori (H. pylori). The aim of this study was to provide further evidence for our previous proposal regarding the occurrence of H.pylori inside the yeast vacuole, verifying the viability of the intravacuolar H.pylori by western blotting. Methods: Light microscopy and polymerase chain reaction (PCR) were used for primary detection of nonculturable H.pylori in 11 Candida yeasts (six oral and five gastric). Boiling was used for extraction of proteins from yeasts and the control H.pylori. Western blot analysis was recruited to assess the occurrence of H.pylori-specific proteins in protein pool of yeasts, using IgY-Hp raised in hens and IgG1-Hp raised in mice. Results: The fast-moving bacterium-like bodies (BLBs) were identified as H.pylori by amplification of H.pylori 16S rRNA, ureAB, vacAs1, and ahpC genes from the whole DNA of yeasts. Analysis of the sequenced products of 16S rRNA gene amplified from the yeast and H.pylori isolates of patient #2 showed 100 % homology with the corresponding sequences of the reference H. pylori strains in GenBank. According to published data, it was plausible to assign the H.pylori-specific proteins, detected by western blot analysis, as thiol peroxidase(21 kDa), peroxiredoxin (AhpC) (26 kDa), urease-A subunit (UreA) (32 kDa), vacuolating cytotoxin A (VacA) small subunit (36 kDa), and vacA large subunit (56 kDa). Conclusion: Results of this study show that inside yeast, H.pylori expresses proteins and is viable. These proteins appear to serve as powerful tools to help H.pylori to establish in the vacuole of yeast where it can reach nutrients and multiply. The intimate relationship between H.pylori and Candida yeast which began long time ago, could have led to the establishment of H.pylori inside the yeast vacuole before invading human cells. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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