An In vitro Study on Chick Somite Ability to Express Cerberus, Chordin, FGF8, Follistatin, and Noggin Transcripts.
Background: In vitro simulation of developmental processes is an invaluable tool to shed light on the intrinsic mechanism of developmental biosystems such as central nervous system in mammals. Chick somites have been used to simulate the neural differentiation of human neural progenitor cells. In th...
| Publicado en: | Avicenna Journal of Medical Biotechnology Vol. 6; no. 2; pp. 119 - 123 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Avicenna Research Institute
Apr-Jun2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=95660829&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 95660829 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20082835 ATX9 jtl: Avicenna Journal of Medical Biotechnology issn: 20082835 maglogo: N pubinfo: dt: Apr-Jun2014 vid: 6 iid: 2 pid: 56911 pub: Avicenna Research Institute artinfo: ui: 95660829 95660829 103934522 95660829 ppf: 119 ppct: 4 formats: tig: atl: An In vitro Study on Chick Somite Ability to Express Cerberus, Chordin, FGF8, Follistatin, and Noggin Transcripts. aug: au: Hosseini Farahabadi, Samaneh Sadat Karbalaie, Khadijeh Salehi, Hossein Rabiee, Farzaneh Ghaedi, Kamran Nasr-Esfahani, Mohammad-Hossein affil: Cell & Molecular Biology Division, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran sug: subj: Proteins Classification Cells Embryo Research Methods Animal Studies In Vitro Studies Birds Polymerase Chain Reaction ab: Background: In vitro simulation of developmental processes is an invaluable tool to shed light on the intrinsic mechanism of developmental biosystems such as central nervous system in mammals. Chick somites have been used to simulate the neural differentiation of human neural progenitor cells. In the present study, we aimed to indicate whether somites have the ability to express required neural differentiation factors at mRNA level. Methods: Chick embryos were isolated from the yolk surface of the fertilized eggs and somites were subsequently isolated from embryos under a dissecting microscope. Total RNA of the somites was extracted and RT-PCR carried out with specific primers of cerberus, chordin, FGF8, follistatin and noggin. Results: Data showed that five aforementioned factors were co-expressed after 7 days in vitro by somites. Conclusion: We concluded that neural induction property of somites appeared by production of required neural differentiation factors including cerberus, chordin, FGF8, follistatin and noggin. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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