Photonic Crystal Kinase Biosensor.
We have developed a novel biosensor for kinases that is based on a kinase-responsive polymer hydrogel, which enables label-free screening of kinase activity via changes in optical properties. The hydrogel is specifically designed to swell reversibly upon phosphorylation of a target peptide, triggeri...
| Publicado en: | Journal of the American Chemical Society Vol. 136; no. 19; pp. 6896 - 6900 |
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| Autores principales: | , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
5/14/2014
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=96326855&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 96326855 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 5/14/2014 vid: 136 iid: 19 pid: 997 pub: American Chemical Society artinfo: ui: 96326855 10.1021/Ja5031062 ppf: 6896 ppct: 4 formats: tig: atl: Photonic Crystal Kinase Biosensor. aug: au: MacConaghy, Kelsey I. Geary, Christopher I. Kaar, Joel L. Stoykovich, Mark P. affil: Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, United States su: Biosensors Machine design Kinases Colloids Chemical reactions Optical properties sug: subj: Biosensors Machine design Kinases Colloids Chemical reactions Optical properties ab: We have developed a novel biosensor for kinases that is based on a kinase-responsive polymer hydrogel, which enables label-free screening of kinase activity via changes in optical properties. The hydrogel is specifically designed to swell reversibly upon phosphorylation of a target peptide, triggering a change in optical diffraction from a crystalline colloidal array of particles impregnated into the hydrogel. Diffraction measurements, and charge staining, confirmed the responsive nature of the hydrogel. Moreover, the change in diffraction of the hydrogel upon treatment with kinase exhibited a time- and dose-dependent response. A theoretical model for ionic polymer networks describes the observed optical response well and can be used to quantify the extent of phosphorylation. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2014 holdings: @attributes: islocal: N |
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