Genetic Diversity among Yersinia enterocolitica Isolated from Chicken and Fish in and around Coimbatore City, India.
Background: Yersinia enterocolitica is rapidly emerging worldwide as an enteric pathogen and has become a major cause of diarrhea even in developed countries. The aim of this study was to characterize and genetic diversity analysis among Y. enterocolitica strains isolated from fish and chicken sourc...
| Publicado en: | Iranian Journal of Public Health Vol. 43; no. 6; pp. 835 - 845 |
|---|---|
| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Tehran University of Medical Sciences
Jun2014
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=97028737&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 97028737 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 22516085 6N6F jtl: Iranian Journal of Public Health issn: 22516085 maglogo: N pubinfo: dt: Jun2014 vid: 43 iid: 6 pid: 21783 pub: Tehran University of Medical Sciences artinfo: ui: 97028737 97028737 103974778 97028737 ppf: 835 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Genetic Diversity among Yersinia enterocolitica Isolated from Chicken and Fish in and around Coimbatore City, India. aug: au: SHANMUGAPRIYA, Seshadhri SENTHILMURUGAN, Thangavel THAYUMANAVAN, Thangavelu affil: School of Biotechnology, Dr. G.R. Damodaran College of Science, India sug: subj: Yersinia Infections Familial and Genetic Fish Analysis Poultry Analysis Human India Descriptive Statistics Polymorphism, Genetic Drug Resistance, Microbial Evaluation ab: Background: Yersinia enterocolitica is rapidly emerging worldwide as an enteric pathogen and has become a major cause of diarrhea even in developed countries. The aim of this study was to characterize and genetic diversity analysis among Y. enterocolitica strains isolated from fish and chicken sources. Methods: From 44 strains, 55% (24 strains) found to be positive for Y. enterocolitica by colony morphology, biochemical tests and 16S rRNA. We investigate the diversity of Y. enterocolitica by hemolytic activity, antimicrobial resistance, RAPD, ERIC and REP-PCRs PCR, profiling of outermembrane proteins and lipopolysaccarides. Results: Forty one percent of the strains were found to be the producers of haemolysin at 37 ºC but not at 28 ºC. All the isolates were exhibiting multi-drug resistance and found sensitive to chloramphenicol, and resistant to ciprofloxacin and amoxicillin. Eight, eleven and twelve different genotypic patterns were observed in RAPD, ERIC and REP-PCRs respectively. Five isolates have produced high molecular weight protein (HMWP) with a molecular weight of 150 - 220 kDa. Mostly LPS produce identical profiles, 22 strains have produced smooth LPS, while 2 strains have produced the rough LPS pattern. Conclusion: Genotyping tools strongly confirm the co-existence wide genetic diversity among the strains tested. By using any or the combination of these molecular tools, epidemiological investigation on Y. enterocolitica could be elucidated effectively. These results showed that the REP-PCR is more informative and discriminative than other for analysis of Y. enterocolitica diversity. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|