Molecular characterization of Cryptococcus gattii genotype AFLP6/VGII isolated from woody debris of divi-divi (Caesalpinia coriaria), Bonaire, Dutch Caribbean.
Background: The basidiomycetous yeast Cryptococcus gattii is an emerging and primary pathogen. There is a lack of information about its environmental spread outside outbreak regions in Mediterranean Europe, North and South America. Environmental sampling for C. gattii and molecular characterization...
| Publicado en: | Revista Iberoamericana de Micologia Vol. 31; no. 3; pp. 193 - 197 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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Elsevier B.V.
7/31/2014
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=99311511&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 99311511 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 11301406 PVP jtl: Revista Iberoamericana de Micologia issn: 11301406 maglogo: N pubinfo: dt: 7/31/2014 vid: 31 iid: 3 pid: 467 pub: Elsevier B.V. artinfo: ui: 99311511 10.1016/j.riam.2013.10.007 ppf: 193 ppct: 4 formats: fmt: @attributes: type: P size: 2.6MB tig: atl: Molecular characterization of Cryptococcus gattii genotype AFLP6/VGII isolated from woody debris of divi-divi (Caesalpinia coriaria), Bonaire, Dutch Caribbean. aug: au: Hagen, Ferry Chowdhary, Anuradha Prakash, Anupam Yntema, Jan-Bart Meis, Jacques F. affil: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmim Hospital, Nijmegen, Netherlands Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, Netherlands Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India Department of Pediatric Pulmonology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands su: Cryptococcus Cryptococcaceae Tree diseases & pests Caesalpinia coriaria Genetic polymorphisms in plants Basidiomycetes sug: subj: Cryptococcus Cryptococcaceae Tree diseases & pests Caesalpinia coriaria Genetic polymorphisms in plants Basidiomycetes keyword: Amplified fragment length polymorphism Cryptococcus gattii Environmental sampling fingerprinting Multi-locus sequence typing amplificados Muestreo medioambiental Polimorfismo de tamaño de fragmentos Tipado por secuenciación de múltiples loci ab: Background: The basidiomycetous yeast Cryptococcus gattii is an emerging and primary pathogen. There is a lack of information about its environmental spread outside outbreak regions in Mediterranean Europe, North and South America. Environmental sampling for C. gattii and molecular characterization of the obtained isolates will provide an insight into the global spread of the various genotypes. Methods: Woody debris of native divi-divi (Caesalpinia coriaria) trees were sampled across Bonaire, Dutch Caribbean. Colonies suspected for Cryptococcus species were subjected to standard mycology investigations and identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Isolates identified as C. gattii were subjected to amplified fragment length polymorphism genotyping, mating-type analysis and multi-locus sequence typing. Results: Ten colonies of C. gattii were cultured from different trunk hollows of the same divi-divi tree. Molecular characterization showed that all isolates were genotype AFLP6/VGII and mating-type α. Multilocus sequence typing revealed that all isolates were genetically indistinguishable from each other. Conclusions: C. gattii is present in the environment of Bonaire, which suggests that this yeast is likely to be present in the environment of other Caribbean islands. Antecedentes: L a levadura Cryptococcus gattii es un basidiomiceto emergente y patógeno primario. Existe poca información acerca de su dispersión en medio ambiente fuera de las regiones con brotes descritos por esta levadura en la Europa mediterránea, Norte y Sur de América. Los muéstreos del medio ambiente para la búsqueda de C. gattii y la caracterización molecular de los aislamientos obtenidos puede proveer de una visión global sobre la dispersión de varios de sus genotipos. Métodos: Se tomaron muestras de residuos de madera de árboles nativos divi-divi (Caesalpinia coriaria) en Bonaire, Caribe Holandés. Las colonias susceptibles de pertenecer a las especies de Cryptococcus se sometieron a un estudio micológico estándar e identificación por espectrometría de masas MALDI-TOF (Matrix-Assisted Laser Desorption Ionization-Time of Flight). Los aislamientos identificados como C. gattii se sometieron a genotipado mediante AFLP (Amplified Fragment Length Polymorphism), obtención del tipo sexual y MLST (Multi-locus Sequence Typing). Resultados: Se obtuvieron diez colonias de C. gattii en el cultivo de nuestras de diferentes agujeros de un mismo árbol divi-divi. La caracterización molecular mostró que todos los aislamientos eran genotipo AFLP6/VGII y tipo sexual α. El tipado mediante MLST reveló que todos los aislamientos eran genéticamente indistinguibles unos de otros. Conclusiones: C. gattii está presente en el medio ambiente de Bonaire, lo que sugiere que esta levadura podría estar presente en el ambiente de otras islas del Caribe. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Iberoamericana de Micologia is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Iberoamericana de Micologia holder: Elsevier B.V. dt: @attributes: year: 2014 holdings: @attributes: islocal: N |
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