A comparative analysis of TLR5 polymorphism and clinical parameters in typhoid patients and asymptomatic typhoid carriers.
Aim: Typhoid fever is a serious health problem in tropical and developing countries. Although asymptomatic typhoid carriers show no clinical signs, they can spread infection to others. This study investigated the Toll like receptor 5 ( TLR5) polymorphism and various clinical parameters in typhoid pa...
| Published in: | Journal of Public Health: From Theory to Practice (2198-1833) Vol. 22; no. 2; pp. 131 - 138 |
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| Main Authors: | , , , , |
| Format: | Article |
| Published: |
Springer Nature
Apr2014
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| Subjects: | |
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ssf&AN=101069806&site=ehost-live header: @attributes: shortDbName: ssf uiTerm: 101069806 longDbName: Social Sciences Full Text (H.W. Wilson) uiTag: AN controlInfo: bkinfo: jinfo: jid: 21981833 NENI jtl: Journal of Public Health: From Theory to Practice (2198-1833) issn: 21981833 maglogo: N pubinfo: dt: Apr2014 vid: 22 iid: 2 pid: 237 pub: Springer Nature artinfo: ui: 101069806 10.1007/s10389-013-0604-5 ppf: 131 ppct: 7 formats: tig: atl: A comparative analysis of TLR5 polymorphism and clinical parameters in typhoid patients and asymptomatic typhoid carriers. aug: au: Senthilkumar, Balakrishnan Sivakumar, Periasamy Madhanraj, Rajendran Senbagam, Duraisamy Illakia, Sivaji affil: Center for Immunology and Microbial Disease, Albany Medical College, Albany 12208-3479 USA Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram 637 408 India su: Analysis of variance Genetics Fecal analysis C-reactive protein Carrier state (Communicable diseases) Chi-squared test Comparative studies Disease susceptibility Enzymes Genetic polymorphisms Polymerase chain reaction Probability theory Statistics Data analysis Typhoid fever Statistical significance Data analysis software Descriptive statistics Leukocyte count Platelet count sug: subj: Analysis of variance Genetics Fecal analysis C-reactive protein Carrier state (Communicable diseases) Chi-squared test Comparative studies Disease susceptibility Enzymes Genetic polymorphisms Polymerase chain reaction Probability theory Statistics Data analysis Typhoid fever Statistical significance Data analysis software Descriptive statistics Leukocyte count Platelet count keyword: CRP Polymorphism TLR5 Total leukocytes Typhoid carrier CRP Polymorphism TLR5 Total leukocytes Typhoid carrier ab: Aim: Typhoid fever is a serious health problem in tropical and developing countries. Although asymptomatic typhoid carriers show no clinical signs, they can spread infection to others. This study investigated the Toll like receptor 5 ( TLR5) polymorphism and various clinical parameters in typhoid patients and asymptomatic typhoid carriers. Subjects and methods: TLR5 gene was amplified in typhoid patients ( n = 15), asymptomatic carriers ( n = 10) and healthy control subjects ( n = 10) by polymerase chain reaction (PCR). A restriction fragment length polymorphism (RFLP) analysis of TLR5 gene was performed with restriction enzyme (Ddel). Clinical parameters such as total leukocyte, platelets, C-reactive protein (CRP), and adenosine de-aminase (ADA) were estimated. A correlative analysis of TLR5 gene polymorphism and clinical parameters was performed. Results: Typhoid fever patients had significantly ( p < 0.05) reduced total leukocyte and platelet counts when compared to asymptomatic carriers and control subjects. Typhoid patients showed significantly ( p < 0.05) reduced ADA activity, while elevated CRP level when compared to carriers and controls. TLR5 gene was amplified in all individuals, but only two typhoid patients showed TLR5 gene polymorphism ( TLR5). There was no significant correlation between TLR5 gene polymorphism and clinical parameters studied in typhoid patients. Conclusion: The TLR5 gene polymorphism seems to be not associated with susceptibility to typhoid fever. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: N holdings: @attributes: islocal: N |
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