Radiolabeled RGD tracer kinetics annotates differential αvβ 3 integrin expression linked to cell intrinsic and vessel expression.
Purpose: The purpose of this paper is to study the association between RGD binding kinetics and αvβ3 integrin receptor density in the complex tumor milieu.Procedures: We assessed αvβ3 in vitro and by (68)Ga-DOTA-[c(RGDfK)]2 positron emission tomography (PET) in tumors with varying αvβ3.Results: Intr...
| Publicado en: | Molecular Imaging & Biology Vol. 16; no. 4; pp. 558 - 567 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Aug2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103833997&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103833997 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Aug2014 vid: 16 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 103833997 103833997 NLM24310722 2012648384 10.1007/s11307-013-0710-3 NLM24310722 103833997 ppf: 558 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Radiolabeled RGD tracer kinetics annotates differential αvβ 3 integrin expression linked to cell intrinsic and vessel expression. aug: au: Alam, Israt S Witney, Timothy H Tomasi, Giampaolo Carroll, Laurence Twyman, Frazer J Nguyen, Quang-Dé Aboagye, Eric O affil: Comprehensive Cancer Imaging Centre, Faculty of Medicine, Imperial College London, London, W12 0NN, UK. sug: subj: Blood Vessels Metabolism Blood Vessels Pathology Organometallic Compounds Diagnostic Use Peptides Diagnostic Use Receptors, Cell Surface Metabolism Animal Studies Antigens, Surface Metabolism Blotting, Western Cell Line, Tumor Female Flow Cytometry Human Immunohistochemistry Kinetics Mice Neoplasms Blood Supply Neoplasms Pathology Neoplasms Radiography Radioisotopes Tomography, Emission-Computed Female ab: Purpose: The purpose of this paper is to study the association between RGD binding kinetics and αvβ3 integrin receptor density in the complex tumor milieu.Procedures: We assessed αvβ3 in vitro and by (68)Ga-DOTA-[c(RGDfK)]2 positron emission tomography (PET) in tumors with varying αvβ3.Results: Intrinsic αvβ3 expression decreased in the order of M21 >>> MDA-MB-231 > M21L in cells. Tumor volume of distribution by PET, V T, was significantly higher in M21 compared to isogenic M21L tumors (0.40 ± 0.01 versus 0.25 ± 0.02; p < 0.01) despite similar microvessel density (MVD) likely due to higher αvβ3. V T for MDA-MB-231 (0.40 ± 0.04) was comparable to M21 despite lower αvβ3 but in keeping with the higher MVD, suggesting superior tracer distribution.Conclusions: This study demonstrates that radioligand binding kinetics of PET data can be used to discriminate tumors with different αvβ3 integrin expression-a key component of the angiogenesis phenotype-in vivo. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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