Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters.
Background: 4-1BB, a member of the TNF receptor superfamily, has a role in various inflammatory pathologies through its interaction with 4-1BB ligand. We previously demonstrated that it participates in initiating and promoting obesity-induced adipose inflammation in a rodent model.Objective: In this...
| Publicado en: | International Journal of Obesity Vol. 38; no. 8; pp. 1075 - 1083 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Aug2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103838357&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103838357 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03070565 DZC jtl: International Journal of Obesity issn: 03070565 maglogo: Y pubinfo: dt: Aug2014 vid: 38 iid: 8 pid: 2579 pub: Springer Nature place: Dordrecht, <Blank> artinfo: ui: 103838357 NLM24281650 2012671206 10.1038/ijo.2013.222 NLM24281650 103838357 ppf: 1075 ppct: 8 formats: tig: atl: Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters. aug: au: Tu, T H Kim, C-S Kang, J-H Nam-Goong, I S Nam, C W Kim, E S Kim, Y I Choi, J I Kawada, T Goto, T Park, T Yoon Park, J H Choi, M-S Yu, R affil: Department of Food Science and Nutrition, University of Ulsan, Ulsan, South Korea. sug: subj: Tumor Necrosis Factor Metabolism Adipocytes Metabolism Adipose Tissue Metabolism Antigens, Surface Metabolism Inflammation Metabolism Obesity Metabolism Adipose Tissue Immunology Body Mass Index Cells, Cultured Enzyme-Linked Immunosorbent Assay Female Human Inflammation Immunology Male Obesity Immunology Obesity Physiopathology Polymerase Chain Reaction Solubility Female Male ab: Background: 4-1BB, a member of the TNF receptor superfamily, has a role in various inflammatory pathologies through its interaction with 4-1BB ligand. We previously demonstrated that it participates in initiating and promoting obesity-induced adipose inflammation in a rodent model.Objective: In this study, we examined whether 4-1BB is related to obesity-induced adipose inflammation and metabolic parameters in humans.Methods: A total of 50 subjects, 25 obese (body mass index (BMI)≥25 kg m(-2)) and 25 lean (BMI<23 kg m(-2)) participated in the study. The levels of 4-1BB transcripts and soluble 4-1BB protein (s4-1BB) in subcutaneous adipose tissue were measured by quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Inflammatory and metabolic parameters were measured by enzymatic analysis and immunoassay.Results: Obese subjects had higher levels of both 4-1BB transcripts and s4-1BB protein in subcutaneous adipose tissue than lean controls, and the levels were correlated with BMI and the expression of inflammatory markers, as well as with serum metabolic parameters. Moreover, s4-1BB was released from human adipocytes, and elicited chemotactic responses from human monocytes/T cells as well as enhancing their inflammatory activity, indicating that it may promote human adipose inflammation.Discussion: Our data demonstrate that elevated levels of 4-1BB transcripts and s4-1BB in adipose tissue are closely associated with obesity-induced inflammation and metabolic dysregulation. They suggest that both 4-1BB transcripts and s4-1BB could serve as novel biomarkers and/or therapeutic targets for obesity-induced inflammation and metabolic syndrome in humans. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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