Virus-like particles containing the tetrameric ectodomain of influenza matrix protein 2 and flagellin induce heterosubtypic protection in mice.
The ectodomain of matrix protein 2 (M2e) is highly conserved among influenza A viruses and can be a promising candidate antigen for a broadly cross-protective vaccine. In this study, a tetrameric M2e (tM2e) and a truncated form of flagellin (tFliC) were coincorporated into virus-like particles (VLPs...
| Published in: | BioMed Research International Vol. 2013; pp. 686549 - 686550 |
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| Main Authors: | , , , , , |
| Format: | research Journal Article |
| Published: |
Wiley-Blackwell
2013
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104090798&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104090798 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104090798 104090798 2012240575 NLM23984396 PMC3745920 104090798 ppf: 686549 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Virus-like particles containing the tetrameric ectodomain of influenza matrix protein 2 and flagellin induce heterosubtypic protection in mice. aug: au: Wang, Li Wang, Ying-Chun Feng, Hao Ahmed, Tamanna Compans, Richard W Wang, Bao-Zhong affil: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, USA. sug: subj: Orthomyxovirus Infections Immunology Orthomyxovirus Infections Prevention and Control Proteins Proteins Immunology Viruses Immunology Virus-Like Particles Animal Studies Antibody Formation Immunology Antigen-Antibody Reactions Immunology Cell Line Cell Membrane Cell Membrane Metabolism Dogs Female Immunity Immunology Immunoglobulins Blood Influenza A Virus Immunology Mice Molecular Structure Orthomyxovirus Infections Orthomyxovirus Infections Blood T Lymphocytes Immunology Female ab: The ectodomain of matrix protein 2 (M2e) is highly conserved among influenza A viruses and can be a promising candidate antigen for a broadly cross-protective vaccine. In this study, a tetrameric M2e (tM2e) and a truncated form of flagellin (tFliC) were coincorporated into virus-like particles (VLPs) to enhance its immunogenicity. Our data showed that the majority of M2e in VLPs was presented as tetramers by introducing a foreign tetramerization motif GCN4. Intranasal immunization with tM2e VLPs significantly enhanced the levels of serum IgG and IgG subclasses compared to soluble M2e (sM2e) in mice. tM2e VLPs also induced higher M2e-specific T-cell and mucosal antibody responses, conferring complete protection against homologous influenza virus infection. The immunogenicity of tM2e VLPs was further enhanced by coincorporation of the membrane-anchored tFliC (tM2e chimeric VLPs) or coadministration with tFliC VLPs as a mixture, but not the soluble flagellin, inducing stronghumoral and cellular immune responses conferring cross-protection against lethal challenge with heterotypic influenza viruses. These results support the development of tM2e chimeric VLPs as universal vaccines and warrant further investigation. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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