Hedgehog signal inhibitors suppress the invasion of human rhabdomyosarcoma cells.
Purpose: In the treatment of rhabdomyosarcoma (RMS), invasion and metastasis remain the most critical determinants of resectability and survival. The objective of this study was to determine whether Hedgehog (Hh) signaling plays a role in the invasion of RMS.Methods: Two kinds of specific Hh signali...
| Published in: | Pediatric Surgery International Vol. 29; no. 11; pp. 1153 - 1159 |
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| Main Authors: | , , , , |
| Format: | research Journal Article |
| Published: |
Springer Nature
Nov2013
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104105371&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104105371 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01790358 O07 jtl: Pediatric Surgery International issn: 01790358 maglogo: N pubinfo: dt: Nov2013 vid: 29 iid: 11 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104105371 NLM23989521 2012341769 10.1007/s00383-013-3369-6 NLM23989521 PMC3824305 104105371 ppf: 1153 ppct: 6 formats: tig: atl: Hedgehog signal inhibitors suppress the invasion of human rhabdomyosarcoma cells. aug: au: Oue, Takaharu Uehara, Shuichiro Yamanaka, Hiroaki Nomura, Motonari Usui, Noriaki affil: Division of Pediatric Surgery, Department of Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan, ooue@pedsurg.med.osaka-u.ac.jp. sug: subj: Terpenes Pharmacodynamics Proteins Metabolism Neoplasm Invasiveness Rhabdomyosarcoma Metabolism Alkaloids Pharmacodynamics Biological Response Modifiers Pharmacodynamics Cell Line, Tumor Cell Movement Disease Progression Proteins Antagonists and Inhibitors Human Rhabdomyosarcoma Drug Therapy Rhabdomyosarcoma Pathology Signal Transduction ab: Purpose: In the treatment of rhabdomyosarcoma (RMS), invasion and metastasis remain the most critical determinants of resectability and survival. The objective of this study was to determine whether Hedgehog (Hh) signaling plays a role in the invasion of RMS.Methods: Two kinds of specific Hh signaling inhibitors, cyclopamine and forskolin, were used to suppress activated Hh signals in three RMS cell lines. The effects of the Hh signaling inhibitors on tumor cell invasion and motility were investigated using Matrigel invasion assays and wound closure assays, respectively.Results: The number of invaded cells counted in six random microscopic fields in the Matrigel chambers was significantly decreased by both cyclopamine and forskolin in every RMS cell line. Furthermore, the wound closure assays revealed that a blockade of the Hh signaling pathway by the Hh inhibitors strongly impairs RMS cell motility, as visualized by the delayed closure of the gaps generated in the cultured cell monolayers of the three RMS cell lines.Conclusions: Both the invasive capacity and motility of RMS cells are significantly suppressed by Hh signaling inhibitors, demonstrating that the Hh pathway plays an important role in the invasion of RMS. Hh inhibitors may provide a new paradigm for the treatment of RMS. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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