Sensitivity of rabbit ventricular action potential and ca (2+) dynamics to small variations in membrane currents and ion diffusion coefficients.
Little is known about how small variations in ionic currents and Ca²¿ and Na¿ diffusion coefficients impact action potential and Ca²¿ dynamics in rabbit ventricular myocytes. We applied sensitivity analysis to quantify the sensitivity of Shannon et al. model (Biophys. J., 2004) to 5%-10% changes in...
| Publicado en: | BioMed Research International Vol. 2013; pp. 565431 - 565432 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104113704&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104113704 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104113704 2012371313 NLM24222910 PMC3814049 104113704 ppf: 565431 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Sensitivity of rabbit ventricular action potential and ca (2+) dynamics to small variations in membrane currents and ion diffusion coefficients. aug: au: Lo, Yuan Hung Peachey, Tom Abramson, David McCulloch, Andrew Michailova, Anushka affil: Department of Bioengineering, PFBH 241, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412, USA. sug: subj: Action Potentials Physiology Calcium Metabolism Signal Transduction Cells Metabolism Animals Calcium Physiology Heart Ventricle Metabolism Ions Cell Physiology Rabbits Sodium Metabolism ab: Little is known about how small variations in ionic currents and Ca²¿ and Na¿ diffusion coefficients impact action potential and Ca²¿ dynamics in rabbit ventricular myocytes. We applied sensitivity analysis to quantify the sensitivity of Shannon et al. model (Biophys. J., 2004) to 5%-10% changes in currents conductance, channels distribution, and ion diffusion in rabbit ventricular cells. We found that action potential duration and Ca²¿ peaks are highly sensitive to 10% increase in L-type Ca²¿ current; moderately influenced by 10% increase in Na¿-Ca²¿ exchanger, Na¿-K¿ pump, rapid delayed and slow transient outward K¿ currents, and Cl¿ background current; insensitive to 10% increases in all other ionic currents and sarcoplasmic reticulum Ca²¿ fluxes. Cell electrical activity is strongly affected by 5% shift of L-type Ca²¿ channels and Na¿-Ca²¿ exchanger in between junctional and submembrane spaces while Ca²¿-activated Cl¿-channel redistribution has the modest effect. Small changes in submembrane and cytosolic diffusion coefficients for Ca²¿, but not in Na¿ transfer, may alter notably myocyte contraction. Our studies highlight the need for more precise measurements and further extending and testing of the Shannon et al. model. Our results demonstrate usefulness of sensitivity analysis to identify specific knowledge gaps and controversies related to ventricular cell electrophysiology and Ca²¿ signaling. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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