A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function.
Background: Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals. Objectives: We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure...
| Publicado en: | Environmental Health Perspectives Vol. 121; no. 6; pp. 717 - 725 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
National Institute of Environmental Health Sciences
Jun2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104183243&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104183243 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00916765 3B5 jtl: Environmental Health Perspectives issn: 00916765 maglogo: N pubinfo: dt: Jun2013 vid: 121 iid: 6 pid: 56539 pub: National Institute of Environmental Health Sciences place: Research Triangle Park, North Carolina artinfo: ui: 104183243 88195098 10.1289/ehp.1206301 NLM23603051 PMC3672921 104183243 ppf: 717 ppct: 8 formats: fmt: @attributes: type: P tig: atl: A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function. aug: au: Allard, Patrick Kleinstreuer, Nicole C. Knudsen, Thomas B. Colaiácovo, Monica P. affil: Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA; Department of Environmental Health Sciences, University of California in Los Angeles, Los Angeles, California, USA; Institute for Society and Genetics, University of California in Los Angeles, Los Angeles, California, USA sug: subj: Nematodes Biological Assay Chromosomes Drug Effects Agrochemicals Administration and Dosage Agrochemicals Adverse Effects Animal Studies Technology, Pharmaceutical Mann-Whitney U Test Confidence Intervals In Vivo Studies Apoptosis Descriptive Statistics Analysis of Variance Relative Risk Rats Funding Source ab: Background: Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals. Objectives: We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure. Methods: We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database. Results: The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression. Conclusions: This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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