Pharmacodynamic evaluation of irinotecan therapy by FDG and FLT PET/CT imaging in a colorectal cancer xenograft model.
Purpose: Longitudinal changes of 3'-[(18) F]fluoro-3'-deoxythymidine (FLT) and 2-deoxy-2-[(18) F]fluoro-D-glucose (FDG) in response to irinotecan therapy in an animal model of colorectal cancer were compared.Procedures: SCID/CB-17 mice with HCT116 tumors were treated with 50 mg/kg irinotecan by intr...
| Publicado en: | Molecular Imaging & Biology Vol. 14; no. 5; pp. 617 - 625 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Oct2012
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104383297&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104383297 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Oct2012 vid: 14 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104383297 NLM22167582 2011754226 10.1007/s11307-011-0529-8 NLM22167582 104383297 ppf: 617 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Pharmacodynamic evaluation of irinotecan therapy by FDG and FLT PET/CT imaging in a colorectal cancer xenograft model. aug: au: Mudd SR Holich KD Voorbach MJ Cole TB Reuter DR Tapang P Bukofzer G Chakravartty A Donawho CK Palma JP Fox GB Day M Luo Y Mudd, Sarah R Holich, Kimberley D Voorbach, Martin J Cole, Todd B Reuter, David R Tapang, Paul Bukofzer, Gail affil: Translational Imaging and Biochemical Biomarkers, Advanced Technology, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL, USA sug: subj: Camptothecin Analogs and Derivatives Colorectal Neoplasms Drug Therapy Colorectal Neoplasms Radiography Deoxyribonucleosides Diagnostic Use Fludeoxyglucose F 18 Diagnostic Use Animal Studies Body Weights and Measures Camptothecin Pharmacodynamics Camptothecin Therapeutic Use Cells Colorectal Neoplasms Pathology Deoxyribonucleosides Pharmacokinetics Female Fludeoxyglucose F 18 Pharmacokinetics Human Immunohistochemistry Mice Female ab: Purpose: Longitudinal changes of 3'-[(18) F]fluoro-3'-deoxythymidine (FLT) and 2-deoxy-2-[(18) F]fluoro-D-glucose (FDG) in response to irinotecan therapy in an animal model of colorectal cancer were compared.Procedures: SCID/CB-17 mice with HCT116 tumors were treated with 50 mg/kg irinotecan by intraperitoneal injection weekly for 3 weeks. FLT and FDG-positron emission tomography (PET) were performed at baseline, the day after each treatment, and 5 days after the first treatment. Proliferation and apoptosis were evaluated by immunohistochemistry (IHC) after day 15 of imaging.Results: Irinotecan treatment resulted in a suppression of tumor growth. Tumor FLT uptake was decreased the day after each treatment but to a lesser extent 5 days after the first treatment. FDG uptake increased the day after each treatment with a continuous increase throughout the experiment. IHC analysis of phospho-H3 and Ki67 confirmed FLT-PET results, indicating a decrease in proliferation the day after the final irinotecan treatment. Increased apoptosis monitored by caspase-3 was observed after day 15 with irinotecan treatment.Conclusions: FLT-PET may be a better method than FDG-PET for assessing treatment response to irinotecan. Changes in imaging occur before changes in tumor volume. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|