CYP2D6 genotype and dextromethorphan hydroxylation phenotype in an Ecuadorian population...Cytochrome P450 2D6
Purpose: Cytochrome P450 2D6 ( CYP2D6) genotypes and the dextromethorphan/dextrorphan (DXM/DXT) metabolic ratio (MR), which is a marker of CYP2D6 activity, were studied in 118 unrelated healthy Ecuadorians. Methods: Genotyping of CYP2D6 was performed by amplification of entire CYP2D6 gene by XL-PCR...
| Publicado en: | European Journal of Clinical Pharmacology Vol. 68; no. 5; pp. 637 - 645 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
May2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104555267&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104555267 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00316970 NP9 jtl: European Journal of Clinical Pharmacology issn: 00316970 maglogo: N pubinfo: dt: May2012 vid: 68 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104555267 74466838 10.1007/s00228-011-1147-8 NLM22083166 104555267 ppf: 637 ppct: 8 formats: fmt: @attributes: type: P tig: atl: CYP2D6 genotype and dextromethorphan hydroxylation phenotype in an Ecuadorian population...Cytochrome P450 2D6 aug: au: Dorado, Pedro Heras, Natalia Machín, Esther Hernández, Francisco Teran, Enrique LLerena, Adrián affil: CICAB Clinical Research Centre, Extremadura University Hospital and Medical School, Badajoz Spain sug: subj: Oxidoreductases Genotype Dextromethorphan Metabolism Phenotype Hydroxylation Human Ecuador Polymerase Chain Reaction Dextromethorphan Blood Descriptive Statistics Chromatography, High Pressure Liquid Male Female Adolescence Adult Students, College Dextrorphan Blood Linear Regression Spearman's Rank Correlation Coefficient Kruskal-Wallis Test Post Hoc Analysis Data Analysis Software Funding Source Confidence Intervals Adolescent: 13-18 years Adult: 19-44 years Male Female ab: Purpose: Cytochrome P450 2D6 ( CYP2D6) genotypes and the dextromethorphan/dextrorphan (DXM/DXT) metabolic ratio (MR), which is a marker of CYP2D6 activity, were studied in 118 unrelated healthy Ecuadorians. Methods: Genotyping of CYP2D6 was performed by amplification of entire CYP2D6 gene by XL-PCR for CYP2D6*5 and multiplication alleles and by real time-PCR for CYP2D6 *2, *3, *4, *6, *10, *17, *29, *35, *41, and copy number. The plasma levels of DXM and its metabolite DXT were determined on a high-performance liquid chromatography-UV system. Results: The proportions of non-functional alleles were 0.4, 10.6, 0.8, 2.1, and 0% for CYP2D6*3, *4, *4 × N, *5, and *6, respectively. Genotypically, only one of the subjects (0.9%) was homozygous for two inactive alleles and phenotypically classified as a poor metabolizer (PM). The MRs (mean ± standard deviation) corresponding to 'activity scores' of 0, 0.5, 1, 1.5, 2, and 2.5 were 10.57 ( n = 1), 1.63 ± 0.35 ( n = 2), 1.16 ± 0.74 ( n = 29), 1.00 ± 0.47 ( n = 8), 1.24 ± 0.82 ( n = 76), and 1.30 ± 0.32 ( n = 2), respectively. Conclusions: Our data suggest that only 1% of subjects of this Ecuadorian population were PMs and that none were phenotypically ultrarapid metabolizers, which is in agreement with previous findings in other Amerindian populations. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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