Effective reduction of the interleukin-1β transcript in osteoarthritis-prone guinea pig chondrocytes via short hairpin RNA mediated RNA interference influences gene expression of mediators implicated in disease pathogenesis.
Objective: To ascertain a viral vector-based short hairpin RNA (shRNA) capable of reducing the interleukin-1β (IL-1β) transcript in osteoarthritis (OA)-prone chondrocytes and detect corresponding changes in the expression patterns of several critical disease mediators.Methods: Cultured chondrocytes...
| Published in: | Osteoarthritis & Cartilage Vol. 19; no. 12; pp. 1449 - 1458 |
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| Main Authors: | , , , |
| Format: | research Journal Article |
| Published: |
Elsevier B.V.
Dec2011
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104608618&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104608618 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10634584 NZV jtl: Osteoarthritis & Cartilage issn: 10634584 maglogo: N pubinfo: dt: Dec2011 vid: 19 iid: 12 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 104608618 104608618 NLM21945742 2011392489 10.1016/j.joca.2011.09.004 NLM21945742 PMC3256924 104608618 ppf: 1449 ppct: 9 formats: tig: atl: Effective reduction of the interleukin-1β transcript in osteoarthritis-prone guinea pig chondrocytes via short hairpin RNA mediated RNA interference influences gene expression of mediators implicated in disease pathogenesis. aug: au: Santangelo KS Bertone AL Santangelo, K S Bertone, A L affil: Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA sug: subj: Arthritis Pathology Chondrocytes Metabolism Genes Interleukin 1 Osteoarthritis Pathology Animal Studies Arthritis Metabolism Cells Guinea Pigs Heterocyclic Compounds Metabolism Inflammation Mediators Metabolism Male Osteoarthritis Metabolism Reverse Transcriptase Polymerase Chain Reaction Methods RNA Viruses Male ab: Objective: To ascertain a viral vector-based short hairpin RNA (shRNA) capable of reducing the interleukin-1β (IL-1β) transcript in osteoarthritis (OA)-prone chondrocytes and detect corresponding changes in the expression patterns of several critical disease mediators.Methods: Cultured chondrocytes from 2-month-old Hartley guinea pigs were screened for reduction of the IL-1β transcript following plasmid-based delivery of U6-driven shRNA sequences. A successful plasmid/shRNA knockdown combination was identified and used to construct an adeno-associated virus serotype 5 (AAV5) vector for further evaluation. Relative real-time reverse transcription polymerase chain reaction (RT-PCR) was used to quantify in vitro transcript changes of IL-1β and an additional nine genes following transduction with this targeting knockdown vector. To validate in vitro findings, this AAV5 vector was injected into one knee, while either an equivalent volume of saline vehicle (three animals) or non-targeting control vector (three animals) were injected into opposite knees. Fold differences and subsequent percent gene expression levels relative to control groups were calculated using the comparative CT (2(-ΔΔCT)) method.Results: Statistically significant decreases in IL-1β expression were achieved by the targeting knockdown vector relative to both the mock-transduced control and non-targeting vector control groups in vitro. Transcript levels of anabolic transforming growth factor-β (TGF-β) were significantly increased by use of this targeting knockdown vector. Transduction with this targeting AAV5 vector also significantly decreased the transcript levels of key inflammatory cytokines [tumor necrosis factor-α (TNF-α), IL-2, IL-8, and IL-12] and catabolic agents [matrix metalloproteinase (MMP)13, MMP2, interferon-γ (IFN-γ), and inducible nitrous oxide synthase (iNOS)] relative to both mock-transduced and non-targeting vector control groups. In vivo application of this targeting knockdown vector resulted in a >50% reduction (P=0.0045) or >90% (P=0.0001) of the IL-1β transcript relative to vehicle-only or non-targeting vector control exposed cartilage, respectively.Conclusions: Successful reduction of the IL-1β transcript was achieved via RNA interference (RNAi) techniques. Importantly, this alteration significantly influenced the transcript levels of several major players involved in OA pathogenesis in the direction of disease modification. Investigations to characterize additional gene expression changes influenced by targeting knockdown AAV5 vector-based diminution of the IL-1β transcript in vivo are warranted. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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