Determination of daidzein and genistein contents in Mangifera fruit.

The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely 'bacang' (Mangifera foetida), 'kuini' (M. odorata) and 'bambangan' (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried out...

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Published in:Malaysian Journal of Nutrition Vol. 14; no. 2; pp. 189 - 199
Main Authors: Khoo HE, Ismail A
Format: research tables/charts Journal Article
Published: Nutrition Society of Malaysia 2008
Online Access:View this record in EBSCOhost
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      jtl: Malaysian Journal of Nutrition
      issn: 1394035X
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      dt: 2008
      vid: 14
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      pid: 49148
      pub: Nutrition Society of Malaysia
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        atl: Determination of daidzein and genistein contents in Mangifera fruit.
      aug:
        au:
          Khoo HE
          Ismail A
        affil: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
      sug:
        subj:
          Fruit Analysis
          Genistein
          Isoflavones
          Chemistry, Analytical
          Chromatography
          Data Analysis Software
          Descriptive Statistics
          Funding Source
          Malaysia
          One-Way Analysis of Variance
          Post Hoc Analysis
          Solutions
          T-Tests
          Time Factors
          Human
      ab: The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely 'bacang' (Mangifera foetida), 'kuini' (M. odorata) and 'bambangan' (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried out at 80°C for 30, 60 and 90 min. HPLC method was performed with a flow rate of 1.00 ml/min using three different separation columns to determine isoflavone contents. The Zorbax Eclipse RP C18 reverse-phase column was found to give the best resolution for isoflavone separation in Mangifera fruits. Moreover, extraction time of 90 mm was found to increase the isoflavone aglycone contents. At optimised condition, 'kuini' had relatively high daidzein (9.4-10.5 mg/100 g) and genistein (1.6-1.7 mg/100 g) contents. Daidzein content of 'bambangan' (8.3-8.7 mg/100 g) was higher than 'bacang', but the genistein content of 'bambangan' (0.4-0.6 mg/100 g) was similar to that of 'bacang' (0.4-0.8 mg/ 100 g). However, there was a variation in daidzein and genistein contents in Mangifera fruits between two geographical locations.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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