Determination of daidzein and genistein contents in Mangifera fruit.
The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely 'bacang' (Mangifera foetida), 'kuini' (M. odorata) and 'bambangan' (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried out...
| Published in: | Malaysian Journal of Nutrition Vol. 14; no. 2; pp. 189 - 199 |
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| Main Authors: | , |
| Format: | research tables/charts Journal Article |
| Published: |
Nutrition Society of Malaysia
2008
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105331413&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105331413 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1394035X 6CKY jtl: Malaysian Journal of Nutrition issn: 1394035X maglogo: N pubinfo: dt: 2008 vid: 14 iid: 2 pid: 49148 pub: Nutrition Society of Malaysia artinfo: ui: 105331413 105331413 2010447929 105331413 ppf: 189 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Determination of daidzein and genistein contents in Mangifera fruit. aug: au: Khoo HE Ismail A affil: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia sug: subj: Fruit Analysis Genistein Isoflavones Chemistry, Analytical Chromatography Data Analysis Software Descriptive Statistics Funding Source Malaysia One-Way Analysis of Variance Post Hoc Analysis Solutions T-Tests Time Factors Human ab: The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely 'bacang' (Mangifera foetida), 'kuini' (M. odorata) and 'bambangan' (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried out at 80°C for 30, 60 and 90 min. HPLC method was performed with a flow rate of 1.00 ml/min using three different separation columns to determine isoflavone contents. The Zorbax Eclipse RP C18 reverse-phase column was found to give the best resolution for isoflavone separation in Mangifera fruits. Moreover, extraction time of 90 mm was found to increase the isoflavone aglycone contents. At optimised condition, 'kuini' had relatively high daidzein (9.4-10.5 mg/100 g) and genistein (1.6-1.7 mg/100 g) contents. Daidzein content of 'bambangan' (8.3-8.7 mg/100 g) was higher than 'bacang', but the genistein content of 'bambangan' (0.4-0.6 mg/100 g) was similar to that of 'bacang' (0.4-0.8 mg/ 100 g). However, there was a variation in daidzein and genistein contents in Mangifera fruits between two geographical locations. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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