Mitochondrial and sarcoplasmic protein changes in hearts from copper-deficient rats: up-regulation of PGC-1alpha transcript and protein as a cause for mitochondrial biogenesis in copper deficiency.
Changes in mitochondrial and sarcoplasmic proteins using proteinomics and Western blotting in hearts from copper-deficient rats were explored in this study. Also, key enzymes that are involved in cardiac energy metabolism via glycolysis and fatty acid oxidation and related transcription factors were...
| Published in: | Journal of Nutritional Biochemistry Vol. 20; no. 10; pp. 823 - 831 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | research Journal Article |
| Published: |
Elsevier B.V.
Oct2009
|
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105430439&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105430439 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09552863 KIG jtl: Journal of Nutritional Biochemistry issn: 09552863 maglogo: N pubinfo: dt: Oct2009 vid: 20 iid: 10 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 105430439 105430439 NLM18993053 2010401395 10.1016/j.jnutbio.2008.08.001 NLM18993053 105430439 ppf: 823 ppct: 8 formats: tig: atl: Mitochondrial and sarcoplasmic protein changes in hearts from copper-deficient rats: up-regulation of PGC-1alpha transcript and protein as a cause for mitochondrial biogenesis in copper deficiency. aug: au: Medeiros DM Jiang Y Klaahsen D Lin D Medeiros, Denis M Jiang, Yu Klaahsen, Darcey Lin, Dingbo affil: Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, USA sug: subj: Biochemical Phenomena Copper Deficiency Cytoplasm Metabolism Mitochondria Metabolism Muscle Proteins Metabolism Proteins Metabolism RNA Animal Studies Blotting, Western DNA Probes Electrophoresis Male Nucleotides Polymerase Chain Reaction Proteins Rats Male ab: Changes in mitochondrial and sarcoplasmic proteins using proteinomics and Western blotting in hearts from copper-deficient rats were explored in this study. Also, key enzymes that are involved in cardiac energy metabolism via glycolysis and fatty acid oxidation and related transcription factors were determined. Rats were fed one of two diets: a copper-adequate diet containing 6 mg Cu/kg diet or a diet with less than 1 mg Cu/kg diet for 5 weeks. Copper deficiency was confirmed by low liver copper levels, decreased hematocrit levels and cardiac hypertrophy. Proteinomic data revealed that of the more than 50 proteins identified from the mitochondrial fraction of heart tissue, six were significantly down-regulated and nine were up-regulated. The proteins that were decreased were beta enolase 3, carbonic anhydrase 2, aldose reductase 1, glutathione peroxidase, muscle creatine kinase and mitochondrial aconitase 2. The proteins that were up-regulated were isocitrate dehydrogenase, dihydrolipoamide dehydrogenase, transferrin, subunit d of ATP synthase, transthyretin, preproapolipoprotein A-1, GRP 75, alpha-B crystalline and heat shock protein alpha. Follow-up Western blots on rate-limiting enzymes in glycolysis (phosphofructose kinase), fatty acid oxidation (medium chain acyl dehydrogenase, peroxisome proliferator-actvator receptor-alpha or PPARalpha) and gluconeogenesis (phosphoenolpyruvate carboxykinase) did not reveal changes in metabolic enzymes. However, a significant increase in peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha protein, as well as the transcript, which increased 2.5-fold, was observed. It would appear that increased mitochondrial biogenesis known to occur in copper deficiency hearts is caused by an increased expression in the master regulator of mitochondrial biogenesis, PGC-1alpha. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|