Transcript analysis of the selenoproteome indicates that dietary selenium requirements of rats based on selenium-regulated selenoprotein mRNA levels are uniformly less than those based on glutathione peroxidase activity.
Dietary selenium (Se) requirements in rats have been based largely upon glutathione peroxidase-1 (Gpx1) enzyme activity and Gpx1 mRNA levels can also be used to determine Se requirements. The identification of the complete selenoprotein proteome suggests that we might identify additional useful mole...
| Publicado en: | Journal of Nutrition Vol. 139; no. 2; pp. 199 - 207 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Feb2009
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105624780&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105624780 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00223166 NUI jtl: Journal of Nutrition issn: 00223166 maglogo: N pubinfo: dt: Feb2009 vid: 139 iid: 2 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 105624780 105624780 NLM19106321 2010168071 10.3945/jn.108.098624 NLM19106321 PMC2635526 105624780 ppf: 199 ppct: 8 formats: tig: atl: Transcript analysis of the selenoproteome indicates that dietary selenium requirements of rats based on selenium-regulated selenoprotein mRNA levels are uniformly less than those based on glutathione peroxidase activity. aug: au: Barnes KM Evenson JK Raines AM Sunde RA Barnes, Kimberly M Evenson, Jacqueline K Raines, Anna M Sunde, Roger A affil: Department of Nutritional Sciences, University of Wisconsin, Madison, WI 53706, USA sug: subj: Nutritional Requirements Oxidoreductases Metabolism Proteins Proteomics RNA Selenium Administration and Dosage Animals Male Rats Selenium Pharmacokinetics Male ab: Dietary selenium (Se) requirements in rats have been based largely upon glutathione peroxidase-1 (Gpx1) enzyme activity and Gpx1 mRNA levels can also be used to determine Se requirements. The identification of the complete selenoprotein proteome suggests that we might identify additional useful molecular biomarkers for assessment of Se status. To characterize Se regulation of the entire rat selenoproteome, weanling male rats were fed a Se-deficient diet (<0.01 microg Se/g) supplemented with graded levels of Se (0-0.8 microg/g diet) for 28 d, Se status was determined by tissue Se concentration and selenoenzyme activity, and selenoprotein mRNA abundance in liver, kidney, and muscle was determined by quantitative real-time-PCR. Tissue Se and selenoenzyme biomarkers indicated that minimal Se requirements were <or=0.1 microg Se/g diet for most biomarkers. Liver Gpx1 mRNA also decreased to <10% of Se-adequate levels, with a minimum Se requirement at 0.07 microg/g diet. Five selenoprotein mRNA in liver, 4 in kidney, and 2 in muscle decreased to <41% of Se-adequate levels, all with minimum Se requirements at <or=0.07 microg/g diet; the majority of selenoprotein mRNA in each tissue were not significantly regulated by Se status, and 1 selenoprotein, selenophosphate synthetase-2, was upregulated in Se-deficient kidney. Plateau breakpoints for all regulated selenoprotein mRNA were very similar, suggesting that 1 underlying mechanism is in play in Se regulation of selenoprotein mRNA. Lastly, we did not find any selenoprotein mRNA that could be used as biomarkers for super-nutritional/anticarcinogenic levels (up to 0.8 microg Se/g diet) of Se. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|