Comparison of two biochemical tests for identification of enterobacteriaceae in clinical isolates in a tertiary-teaching hospital in Malaysia.
Introduction: Enterobacteriaceae accounts for approximately 80% of clinically significant isolates of Gram-negative bacteria. A relatively simple, rapid method but highly specific is the method of choice for routine identification of Enterobacteriaceae. An accurate result of bacterial identification...
| Publicado en: | International Medical Journal Vol. 15; no. 5; pp. 379 - 384 |
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| Autores principales: | , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Japan International Cultural Exchange Foundation
Dec2008
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105628805&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105628805 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13412051 23J1 jtl: International Medical Journal issn: 13412051 maglogo: N pubinfo: dt: Dec2008 vid: 15 iid: 5 pid: 29386 pub: Japan International Cultural Exchange Foundation artinfo: ui: 105628805 105628805 2010168800 105628805 ppf: 379 ppct: 5 formats: fmt: @attributes: type: P tig: atl: Comparison of two biochemical tests for identification of enterobacteriaceae in clinical isolates in a tertiary-teaching hospital in Malaysia. aug: au: Ismail N Habsah H affil: Department of Medical Microbiology and Parasitology, School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia sug: subj: Bacterial Infections Diagnosis Diagnosis, Laboratory Methods Gram-Negative Bacteria Malaysia Academic Medical Centers Bacterial Infections Epidemiology Chi Square Test Cross Sectional Studies Data Analysis Software Descriptive Research Descriptive Statistics Fisher's Exact Test Malaysia Pearson's Correlation Coefficient Human ab: Introduction: Enterobacteriaceae accounts for approximately 80% of clinically significant isolates of Gram-negative bacteria. A relatively simple, rapid method but highly specific is the method of choice for routine identification of Enterobacteriaceae. An accurate result of bacterial identification is crucial to aid in patient's treatment. The aim of this study was to compare the correct identification of Enterobacteriaceae up to the genus and species level between conventional biochemical tests (CBT) and Enterotube II (ET II). Analytical Profile Index 20E (API 20E) was used as the gold standard test. Methodology: This is a cross sectional descriptive study. A total of 204 isolates were collected from various clinical specimens from Hospital Universiti Sains Malaysia (HUSM) during twelve-month study period from January 2005 to December 2005. All isolates were identified by CBT, ET H and API 20E. Results: The correct identification rate of Enterobacteriaceae among CBT, ET [I and API 20E were 82.6%, 88.7% and 95.6% respectively. Similar results for identification up to the genus level by the three methods were 98.5%. In the analysis of biochemical panels used in API 20E, positive lysine decarboxylase (LDC) test for Escherichia co/i and positive urease test for Klebsieila pneulnoniae were significant (p < 0.05). Co,zclusion: The performance of CBT for the identification of Enferobacteriaceae up to the species level was not as good as ET II and API 20E, but the CBT comparably gave excellent identification up to the genus level. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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