Immunoproteasome in Macaca fascicularis: no age-dependent modification of abundance and activity in the brain and insight into an in silico structural model.
In this study, we investigated proteasome composition and activity in the brain of Macaca fascicularis, in order to test whether this nonhuman primate species might be a suitable animal model for anti-aging therapies in the central nervous system, addressed to the ubiquitin-proteasome system. We det...
| Publicado en: | Rejuvenation Research Vol. 11; no. 1; pp. 73 - 83 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Mary Ann Liebert, Inc.
Feb2008
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105759251&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105759251 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15491684 1E3T jtl: Rejuvenation Research issn: 15491684 maglogo: N pubinfo: dt: Feb2008 vid: 11 iid: 1 pid: 1365 pub: Mary Ann Liebert, Inc. place: New Rochelle, New York artinfo: ui: 105759251 105759251 2009954518 10.1089/rej.2007.0559 NLM17985946 105759251 ppf: 73 ppct: 10 formats: tig: atl: Immunoproteasome in Macaca fascicularis: no age-dependent modification of abundance and activity in the brain and insight into an in silico structural model. aug: au: Bellavista E Mishto M Santoro A Bertoni-Freddari C Sessions RB Franceschi C affil: Interdepartmental Center for Studies on Biophysics, Bioinformatics and Biocomplexity L. Galvani (CIG), University of Bologna, Bologna, Italy. elena.bellavista2@unibo.it sug: subj: Aging Physiology Brain Metabolism Models, Theoretical Peptide Hydrolases Metabolism Peptide Hydrolases Aging Animals Antibodies Pharmacodynamics Brain Physiology Genetic Techniques Immunoproteins Immunology Immunoproteins Metabolism Immunoproteins Physiology Primates Proteins Metabolism Proteins Animal Studies ab: In this study, we investigated proteasome composition and activity in the brain of Macaca fascicularis, in order to test whether this nonhuman primate species might be a suitable animal model for anti-aging therapies in the central nervous system, addressed to the ubiquitin-proteasome system. We detected the catalytic beta subunits of constitutive proteasome, as well as the PA28 regulator and a subunit of immunoproteasome (i.e., beta1i [LMP2]), in seven adult, six old, and one young nonhuman primate brains. Subunit expression and proteasome activity were not influenced by the age of the animal in any of the brain regions (temporal and frontal cortex and cerebellum) we studied. However, an area-specific susceptibility to aged-related oxidative stress emerged. On the whole, the results suggest that, compared to humans, Macaca fascicularis primates may have a different age-dependent regulation of the ubiquitin-proteasome system and, possibly, of neuroinflammation in the brain. An in silico model of the 20S immunoproteasome containing the Macaca fascicularis alpha and beta subunits, present in database or identified by our group (i.e., LMP2), has been developed. Additional information was obtained by de novo sequencing of the beta1 (delta) subunit of Macaca fascicularis. A comparison with humans suggests that in multiprotein complexes some functional subunits, such as alpha subunits, appear to be preferentially conserved during evolution. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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