Identification of HLA-B27-restricted peptides in reactive arthritis and other spondyloarthropathies: computer algorithms and fluorescent activated cell sorting analysis as tools for hunting of HLA-B27-restricted chlamydial and autologous crossreactive peptides involved in reactive arthritis and ankylosing spondylitis.
The illustrated clinical and experimental results demonstrate the strong relationship between the MHC class I antigen HLA-B27 and synovial CD8+ T cells with specificity for bacterial and possible self-antigen in SpA. These new aspects obtained in recent experimental and clinical studies might also p...
| Publicado en: | Rheumatic Disease Clinics of North America Vol. 29; no. 3; pp. 595 - 612 |
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| Autores principales: | , |
| Formato: | Journal Article |
| Publicado: |
W B Saunders
2003 Aug
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105978371&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105978371 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0889857X 4GH jtl: Rheumatic Disease Clinics of North America issn: 0889857X maglogo: N pubinfo: dt: 2003 Aug vid: 29 iid: 3 pid: 1351 pub: W B Saunders place: Philadelphia, Pennsylvania artinfo: ui: 105978371 2009787068 NLM12951870 105978371 ppf: 595 ppct: 17 formats: tig: atl: Identification of HLA-B27-restricted peptides in reactive arthritis and other spondyloarthropathies: computer algorithms and fluorescent activated cell sorting analysis as tools for hunting of HLA-B27-restricted chlamydial and autologous crossreactive peptides involved in reactive arthritis and ankylosing spondylitis. aug: au: Kuon W Sieper J sug: subj: Chlamydia Infections Complications Flow Cytometry Methods HLA Antigens Analysis Reactive Arthritis Immunology Reactive Arthritis Microbiology Algorithms Antigen-Antibody Reactions Chlamydia Infections Immunology HLA Antigens Immunology Proteins Analysis Proteins Immunology Software Spondylarthropathies Immunology Spondylarthropathies Microbiology ab: The illustrated clinical and experimental results demonstrate the strong relationship between the MHC class I antigen HLA-B27 and synovial CD8+ T cells with specificity for bacterial and possible self-antigen in SpA. These new aspects obtained in recent experimental and clinical studies might also provide clues to the pathomechanisms of joint inflammation in SpA. In particular, the newly developed techniques will be of great relevance in the near future. New and more precise bioalgorithms reflecting new insights in the biology and biochemistry of proteins as recently presented [98, 99] can be helpful (e.g., a program with an improved prediction of the features of immunoproteasomes). Intracellular and secreted cytokine staining by FACScan allows examination of a great number of cells expressing certain antigens in response to certain stimuli. The analysis of T-cell responses with tetramer/peptide complexes can be useful to screen tissue sections for TCR, recognizing foreign or self-derived epitopes on those complexes loaded with selected (e.g., bacterial) peptides. Identification of arthritogenic peptides and a further understanding of the immunology of the pathomechanisms in SpA might open ways to design new peptide vaccines to prevent inflammation, autoimmunity, and other diseases by early intervention [100].Copyright © 2003 by Elsevier Inc. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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