| Sumario: | This research was focused on testing a miniature biosensor device that measures amperometric response using a flow-injection immunoassay system for last, quantitative detection of low concentrations of influenza viruses in water samples. The goal was to achieve extremely high selectivity and sensitivity while maintaining fast and user-friendly operation. Based on data obtained previously, the authors explored a flow-injection amperometric immunoassay system that was developed in the University of New Mexico laboratory for hantavirus and bacteria and was adapted for detection of influenza A virus and para influenza virus. Results indicated that both viruses in concentrations as low as 0.5 ngmL can be detected. The higher detection limit can be 300 ng/mL for influenza A and more than 10[4]ng/mL for para influenza. Results indicated that the sandwich scheme assay performed much better than the competitive scheme assay, with a low detection limit of about 5 nM for the competitive scheme compared with 8 pM for the sandwich scheme for rabbit IgG. Five milligrams of graphite powder immunosorbent and 0.5 mM of hydrogen peroxide were the optimum concentrations of immunoassay for anti-rabbit IgG. No interferenee from influenza A virus to para influenza assay was found. In contrast, when immunoassay response for detection of para influenza using influenza A immunosorbent was conducted, it was found that para influenza interferes with influenza A assay.
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