Detection of para influenza and influenza A viruses using flow-injection amperometric immunosensor.
This research was focused on testing a miniature biosensor device that measures amperometric response using a flow-injection immunoassay system for last, quantitative detection of low concentrations of influenza viruses in water samples. The goal was to achieve extremely high selectivity and sensiti...
| Publicado en: | Journal of Applied Research Vol. 7; no. 1; pp. 108 - 127 |
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| Autores principales: | , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Therapeutic Solutions LLC
2007
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106152183&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106152183 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1537064X I4L jtl: Journal of Applied Research issn: 1537064X maglogo: N pubinfo: dt: 2007 vid: 7 iid: 1 pid: 13467 pub: Therapeutic Solutions LLC place: Newtown, Pennsylvania artinfo: ui: 106152183 106152183 2009589888 106152183 ppf: 108 ppct: 19 formats: fmt: @attributes: type: P tig: atl: Detection of para influenza and influenza A viruses using flow-injection amperometric immunosensor. aug: au: Sitdikov RA Wilkins E affil: Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico. sug: subj: Biosensors Trends Haemophilus Infections Prevention and Control Haemophilus Analysis Influenza Prevention and Control Data Analysis, Computer Assisted Data Collection, Computer Assisted Disease Surveillance Methods Electrochemistry Evolution Gene Expression Genetic Research Genotype Haemophilus Infections Diagnosis Immunoassay Immunosorbent Techniques In Vitro Studies Influenza Diagnosis Membrane Proteins Analysis New Mexico Nucleic Acid Hybridization Receptors, Cell Surface Analysis Sequence Analysis Tissue Culture Techniques Virulence Human ab: This research was focused on testing a miniature biosensor device that measures amperometric response using a flow-injection immunoassay system for last, quantitative detection of low concentrations of influenza viruses in water samples. The goal was to achieve extremely high selectivity and sensitivity while maintaining fast and user-friendly operation. Based on data obtained previously, the authors explored a flow-injection amperometric immunoassay system that was developed in the University of New Mexico laboratory for hantavirus and bacteria and was adapted for detection of influenza A virus and para influenza virus. Results indicated that both viruses in concentrations as low as 0.5 ngmL can be detected. The higher detection limit can be 300 ng/mL for influenza A and more than 10[4]ng/mL for para influenza. Results indicated that the sandwich scheme assay performed much better than the competitive scheme assay, with a low detection limit of about 5 nM for the competitive scheme compared with 8 pM for the sandwich scheme for rabbit IgG. Five milligrams of graphite powder immunosorbent and 0.5 mM of hydrogen peroxide were the optimum concentrations of immunoassay for anti-rabbit IgG. No interferenee from influenza A virus to para influenza assay was found. In contrast, when immunoassay response for detection of para influenza using influenza A immunosorbent was conducted, it was found that para influenza interferes with influenza A assay. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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