Sciatic inflammatory neuritis: a non-surgical technique.
Many animal models of neuropathic pain involve both surgery and neural trauma. Chacur et al described the surgical placement of perisciatic gelfoam and a cannula through which zymosan was introduced after healing. We report a simpler percutaneous technique by which to deliver zymosan to the sciatic...
| Publicado en: | Journal of Neuropathic Pain & Symptom Palliation Vol. 1; no. 4; pp. 3 - 12 |
|---|---|
| Autores principales: | , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2005
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| Sumario: | Many animal models of neuropathic pain involve both surgery and neural trauma. Chacur et al described the surgical placement of perisciatic gelfoam and a cannula through which zymosan was introduced after healing. We report a simpler percutaneous technique by which to deliver zymosan to the sciatic nerve and in the rat. Using a nerve stimulator we located the sciatic nerve and delivered either zymosan in incomplete Freunds (IFA) or IFA alone. Animals were tested for tactile allodynia at baseline and for up to 12 days post-treatment. Mean baseline withdrawal values did not differ between groups. Zymosan-treated rats showed decreased paw withdrawal thresholds (PWT) from day 3 [day 0: 14.3 +/- 0.52 g; day 3: 8.5 +/- 0.42 g; p < 0.05]. At day 12 there was bilateral tactile allodynia in animals that received zymosan [ipsilateral PWT: 4.9 +/- 1.01 g; contralateral: 7.2 +/- 1.35 g]. Statistically significant changes were observed only for zymosan-treated rats. We extended the observations to approximately 70 days, at which time allodynia was still robust. Both astrocyte activation and IL-1â expression in the ipsilateral spinal cord of persistently allodynic rats appeared to be greater than that in their counterparts that had recovered. This approach represents a simple, non-surgical procedure by which to induce chronic inflammatory neuritis. It thus lends itself to the study of both chronic pain and the prodromal phase. |
|---|