Oral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis.
BACKGROUND: The development of an efficacious vaccine against infection with Helicobacter pylori, the causative agent of chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma, remains a challenge. Since the use of mucosal adjuvants is limited in human application, we have evaluated the...
| Publicado en: | Journal of Infectious Diseases Vol. 192; no. 8; pp. 1441 - 1450 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
10/15/2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106342805&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106342805 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00221899 1YU jtl: Journal of Infectious Diseases issn: 00221899 maglogo: N pubinfo: dt: 10/15/2005 vid: 192 iid: 8 pid: 622 pub: Oxford University Press / USA artinfo: ui: 106342805 106342805 2009283624 10.1086/444425 NLM16170763 106342805 ppf: 1441 ppct: 9 formats: tig: atl: Oral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis. aug: au: Corthésy B Boris S Isler P Grangette C Mercenier A affil: R&D Laboratory of the Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne Switzerland sug: subj: Bacterial Vaccines Administration and Dosage Helicobacter Infections Prevention and Control Helicobacter Pylori Drug Effects Administration, Oral Animal Studies Antibodies Blood Antibody Formation Antigens Bacterial Vaccines Immunology Bacterial Vaccines Pharmacodynamics Blotting, Western Cytokines Descriptive Statistics DNA Analysis Funding Source Helicobacter Infections Immunology Helicobacter Infections Physiopathology Immunoglobulins Immunoglobulins Blood Intestinal Mucosa Drug Effects Lactobacillus Mann-Whitney U Test Mice Models, Biological Polymerase Chain Reaction Recombinant Proteins Administration and Dosage Recombinant Proteins Immunology T-Tests Toxins Tumor Necrosis Factor Urease ab: BACKGROUND: The development of an efficacious vaccine against infection with Helicobacter pylori, the causative agent of chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma, remains a challenge. Since the use of mucosal adjuvants is limited in human application, we have evaluated the potential of recombinant Lactobacillus strains producing H. pylori urease B (UreB) subunit to deliver this antigen to the gastrointestinal tract. METHODS: Mice were injected orally 3 times with a triple dose of recombinant Lactobacillus plantarum NCIMB8826, the recombinant isogenic cell-wall mutant (alr(-) MD007 strain) expressing UreB, or a mixture of recombinant UreB and cholera toxin (rUreB/CT) as a control. Urease-specific seric immunoglobulin (Ig) G and IgA were measured by use of an enzyme-linked immunosorbent assay. After challenge with Helicobacter felis, stomach infection was examined by use of the rapid urease test and by polymerase chain reaction detection of Helicobacter genomic DNA. RESULTS: Intragastric immunization with both recombinant Lactobacillus strains and rUreB/CT elicited UreB-specific antibodies. After challenge, reduction of H. felis load in the stomachs of mice was observed only after immunization with the recombinant mutant strain MD007 or with rUreB/CT. CONCLUSIONS: This is the first report of successful induction of partial protection against H. felis with a mucosal prime-boost regimen in which recombinant Lactobacillus strains were used as antigen-delivery vehicles. Copyright © 2005 Infectious Diseases Society of America pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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