Assessment of chemosensitivity by monitoring bcl-2 transcript kinetics in acute myeloid leukemias.
Enforced bcl-2 gene expression suppresses apoptosis and confers resistance to anticancer drugs. Here we established a real time fluorescence PCR assay to analyze the association between the bcl-2 gene expression and clinical chemosensitivity in acute myeloid leukemia. Expression levels of the bcl-2...
| Publicado en: | Hematological Oncology Vol. 23; no. 2; pp. 68 - 73 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jun2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| Sumario: | Enforced bcl-2 gene expression suppresses apoptosis and confers resistance to anticancer drugs. Here we established a real time fluorescence PCR assay to analyze the association between the bcl-2 gene expression and clinical chemosensitivity in acute myeloid leukemia. Expression levels of the bcl-2 gene were measured and normalized by beta-actin, a housekeeping gene expressed as endogenous reference. By applying real time PCR to clinical samples, we observed that although the bcl-2/beta-actin ratio was not related to FAB subtypes, the changing data following remission induction therapy clearly reflected drug-sensitivity. These results suggest that RT-PCR assay monitored the efficacy of the chemotherapy by quantifying the bcl-2 gene transcript in AML. |
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