Assessment of chemosensitivity by monitoring bcl-2 transcript kinetics in acute myeloid leukemias.
Enforced bcl-2 gene expression suppresses apoptosis and confers resistance to anticancer drugs. Here we established a real time fluorescence PCR assay to analyze the association between the bcl-2 gene expression and clinical chemosensitivity in acute myeloid leukemia. Expression levels of the bcl-2...
| Publicado en: | Hematological Oncology Vol. 23; no. 2; pp. 68 - 73 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jun2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106426501&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106426501 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02780232 O85 jtl: Hematological Oncology issn: 02780232 maglogo: Y pubinfo: dt: Jun2005 vid: 23 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 106426501 2009145292 10.1002/hon.746 NLM16178036 106426501 ppf: 68 ppct: 5 formats: tig: atl: Assessment of chemosensitivity by monitoring bcl-2 transcript kinetics in acute myeloid leukemias. aug: au: Liu K Kreuzer K Lass U Schmidt CA affil: Department of Pathology, Medical College, Jinan University, Guangzhou, Guangdong 510632, China; tlksh@jnu.edu.cn sug: subj: Apoptosis Chemotherapy, Cancer Evaluation Drug Resistance, Neoplasm Leukemia, Myeloid Antineoplastic Agents Therapeutic Use Data Analysis Software DNA Analysis Electrophoresis Funding Source Genetic Techniques Leukemia, Myeloid Drug Therapy Leukemia, Myeloid Immunology Leukemia, Myeloid Physiopathology Leukemia, Myeloid Prognosis Polymerase Chain Reaction Methods Survival Human ab: Enforced bcl-2 gene expression suppresses apoptosis and confers resistance to anticancer drugs. Here we established a real time fluorescence PCR assay to analyze the association between the bcl-2 gene expression and clinical chemosensitivity in acute myeloid leukemia. Expression levels of the bcl-2 gene were measured and normalized by beta-actin, a housekeeping gene expressed as endogenous reference. By applying real time PCR to clinical samples, we observed that although the bcl-2/beta-actin ratio was not related to FAB subtypes, the changing data following remission induction therapy clearly reflected drug-sensitivity. These results suggest that RT-PCR assay monitored the efficacy of the chemotherapy by quantifying the bcl-2 gene transcript in AML. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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