Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda.

Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the...

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Detalles Bibliográficos
Publicado en:	BioMed Research International Vol. 2015; pp. 1 - 10
Autores principales:	Mugizi, Denis Rwabiita, Muradrasoli, Shaman, Boqvist, Sofia, Erume, Joseph, Nasinyama, George William, Waiswa, Charles, Mboowa, Gerald, Klint, Markus, Magnusson, Ulf
Formato:	pictorial research tables/charts Journal Article
Publicado:	Wiley-Blackwell 2/22/2015
Acceso en línea:	Ver este registro en EBSCOhost

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Sumario:	Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.