Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda.
Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the...
| Publicado en: | BioMed Research International Vol. 2015; pp. 1 - 10 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/22/2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109273431&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109273431 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/22/2015 vid: 2015 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109273431 109273431 109273431 10.1155/2015/720413 109273431 ppf: 1 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda. aug: au: Mugizi, Denis Rwabiita Muradrasoli, Shaman Boqvist, Sofia Erume, Joseph Nasinyama, George William Waiswa, Charles Mboowa, Gerald Klint, Markus Magnusson, Ulf affil: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda sug: subj: Cattle Milk Analysis Brucellosis Etiology Genotype Phenotype Molecular Biology Methods Uganda DNA Analysis Polymerase Chain Reaction Descriptive Statistics Electrophoresis, Agar Gel Sequence Analysis Bacterial Typing Techniques Funding Source ab: Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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