| Sumario: | Ethanol extract of the aerial parts ofBergenia ligulatawas subjected to solvent–solvent separation followed by various chromatographic techniques that lead to isolation of bergenine (1),p-hydroxybenzoyl bergenin (2), 11-O-galloylbergenin (3) and methyl gallate (4) as major constituents. Ethyl acetate fraction showed a dose-dependent urease inhibitory pattern with IC50value of 54μg/mL. Structures of compounds1and3were established by XRD and2,4by NMR. All these compounds were subjected to DPPH scavenging activity, reducing power assay and urease inhibitory activity. The EC507.45 ± 0.2 μg/mL and 5.39 ± 0.28 μg/mL values in terms of antioxidant and reducing power, respectively, were less for3. Compounds1–3showed moderate to significant urease inhibitory potential with IC5057.1 ± 0.7, IC5048.4 ± 0.3 and 38.6 ± 1.5. Antioxidant activities and urease inhibitory potential were investigated and compound3was found to be the most active.
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