Effects of low-stearate palm oil and high-stearate lard high-fat diets on rat liver lipid metabolism and glucose tolerance.
Background: Excess consumption of energy-dense, high-fat Western diets contributes to the development of obesity and obesity-related disorders, such as fatty liver disease. However, not only the quantity but also the composition of dietary fat may play a role in the development of liver steatosis. T...
| Published in: | Nutrition & Metabolism pp. 1 - 12 |
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| Main Authors: | , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
BioMed Central
12/18/2015
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=111874137&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 111874137 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17437075 1CYX jtl: Nutrition & Metabolism issn: 17437075 maglogo: N pubinfo: dt: 12/18/2015 pid: 24147 pub: BioMed Central artinfo: ui: 111874137 111874137 111874137 10.1186/s12986-015-0053-y 111874137 ppf: 1 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Effects of low-stearate palm oil and high-stearate lard high-fat diets on rat liver lipid metabolism and glucose tolerance. aug: au: Janssens, Sharon Heemskerk, Mattijs M. van den Berg, Sjoerd A. van Riel, Natal A. Nicolay, Klaas van Dijk, Ko Willems Prompers, Jeanine J. affil: Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands sug: subj: Oils Metabolism Fatty Acids Metabolism Dietary Fats Metabolism Lipids Metabolism Liver Metabolism Glucose Metabolism Animal Studies Rats In Vivo Studies Glucose Tolerance Test Magnetic Resonance Spectroscopy Methods Mitochondria Analysis Lipoproteins, LDL Oxygen Consumption Descriptive Statistics Data Analysis Software Analysis of Variance Paired T-Tests Post Hoc Analysis Body Weight Changes Blood Glucose ab: Background: Excess consumption of energy-dense, high-fat Western diets contributes to the development of obesity and obesity-related disorders, such as fatty liver disease. However, not only the quantity but also the composition of dietary fat may play a role in the development of liver steatosis. The aim of this study was to determine the effects of low-stearate palm oil and high-stearate lard high-fat diets on in vivo liver lipid metabolism. Methods: Wistar rats were fed with either normal chow (CON), a high-fat diet based on palm oil (HFP), or a high-fat diet based on lard (HFL). After 10 weeks of diet, magnetic resonance spectroscopy was applied for the in vivo determination of intrahepatocellular lipid content and the uptake and turnover of dietary fat after oral administration of 13C-labeled lipids. Derangements in liver lipid metabolism were further assessed by measuring hepatic very-low density lipoprotein (VLDL) secretion and ex vivo respiratory capacity of liver mitochondria using fat-derived substrates. In addition, whole-body and hepatic glucose tolerance were determined with an intraperitoneal glucose tolerance test. Results: Both high-fat diets induced liver lipid accumulation (p < 0.001), which was accompanied by a delayed uptake and/or slower turnover of dietary fat in the liver (p < 0.01), but without any change in VLDL secretion rates. Surprisingly, liver lipid content was higher in HFP than in HFL (p < 0.05), despite the increased fatty acid oxidative capacity in isolated liver mitochondria of HFP animals (p < 0.05). In contrast, while both high-fat diets induced whole-body glucose intolerance, only HFL impaired hepatic glucose tolerance. Conclusion: High-fat diets based on palm oil and lard similarly impair the handling of dietary lipids in the liver, but only the high-fat lard diet induces hepatic glucose intolerance. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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