| Sumario: | Fullerenes (C) are some of the most promising carbon nanomaterials to be used for medical applications as drug delivery agents. Computational and experimental studies have proposed their ability to enter cells by penetrating lipid bilayers. The aim of our study was to provide experimental evidence on whether pristine C in physiological media could penetrate cell membranes. The effect was tested on phospholipid vesicles (liposomes) composed of 1,2-dipalmitoyl- sn-glycero-3-phosphocholine, and validated on isolated human red blood cells (RBCs). We incubated the liposomes in an aqueous suspension of C and dissolved the lipids and C together in chloroform and subsequently formatted the liposomes. By differential scanning calorimetry measurements, we assessed the effect of C on the phospholipid thermal profile. The latter was not affected after the incubation of liposomes in the C suspension; also, a shape transformation of RBCs did not occur. Differently, by dispersing both C and the phospholipids in chloroform, we confirmed the possible interaction of C with the bilayer. We provide experimental data suggesting that the suspension medium is an important factor in determining the C-membrane interaction, which is not always included in computational studies. Since the primary particle size is not the only crucial parameter in C-membrane interactions, it is important to determine the most relevant characteristics of their effects on membranes.
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