Biodistribution Study of Intravenously Injected Cetuximab-IRDye700DX in Cynomolgus Macaques.
Purpose: The use of receptor-targeted antibodies conjugated to photosensitizers is actively being explored to enhance treatment efficacy. To facilitate clinical testing, we evaluated cetuximab conjugated to IRDye700DX (IR700) in cynomolgus macaques.Procedures: Total IR700 and intact cetuximab-IR700...
| Publicado en: | Molecular Imaging & Biology Vol. 18; no. 2; pp. 232 - 243 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Apr2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=113577012&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 113577012 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Apr2016 vid: 18 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 113577012 113577012 NLM26335283 113577012 10.1007/s11307-015-0892-y NLM26335283 113577012 ppf: 232 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Biodistribution Study of Intravenously Injected Cetuximab-IRDye700DX in Cynomolgus Macaques. aug: au: Boer, E. Samuel, S. French, D. Warram, J. Schoeb, T. Rosenthal, E. Zinn, K. de Boer, E French, D N Warram, J M Schoeb, T R Rosenthal, E L Zinn, K R affil: Department of Radiology, University of Alabama at Birmingham, Volker Hall G082, 1670 University Boulevard Birmingham 35294 USA sug: subj: Molecular Imaging Methods Dyes Metabolism Light Male Injections, Intravenous Primates Female Animal Studies Validation Studies Comparative Studies Evaluation Research Multicenter Studies Scales Male Female ab: Purpose: The use of receptor-targeted antibodies conjugated to photosensitizers is actively being explored to enhance treatment efficacy. To facilitate clinical testing, we evaluated cetuximab conjugated to IRDye700DX (IR700) in cynomolgus macaques.Procedures: Total IR700 and intact cetuximab-IR700 were measured in 51 tissues at 2 and 14 days after intravenous injection of 40 and 80 mg/kg cetuximab-IR700, respectively, and compared with an unlabeled cetuximab-dosed control group (two each per sex per time point per group).Results: The IR700 retrieved from all tissues at 2 and 14 days after dosing was estimated at 34.9 ± 1.8 and 2.53 ± 0.67% of the total dose, respectively. The tissues with the highest levels of intact cetuximab-IR700 at 2 days after dosing were the blood, lung, and skin. Formalin-fixed paraffin-embedded tissue sections at 2 days after dosing showed the highest IR700 signals in the axillary lymph node, mammary gland, and gall bladder.Conclusions: Both IR700 and intact cetuximab-IR700 biodistributions were consistent with known epidermal growth factor receptor (EGFR) expression, and changes between 2 and 14 days were consistent with rapid metabolism and excretion of the cetuximab-IR700. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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