Latent herpes simplex virus in human trigeminal ganglia. Detection of an immediate early gene "anti-sense" transcript by in situ hybridization.
We used in situ hybridization to study the expression of herpes simplex virus type 1 genes during latent infections of human sensory ganglia. Trigeminal ganglia were recovered at autopsy from 24 subjects with no evidence of an active herpetic infection. These ganglia were hybridized to 35S-labeled s...
| Publicado en: | New England Journal of Medicine Vol. 317; no. 23; pp. 1427 - 1433 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
New England Journal of Medicine
12/3/87
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119722332&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119722332 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00284793 NEJ jtl: New England Journal of Medicine issn: 00284793 maglogo: N pubinfo: dt: 12/3/87 vid: 317 iid: 23 pid: 388 pub: New England Journal of Medicine place: Waltham, Massachusetts artinfo: ui: 119722332 119722332 NLM2825014 119722332 NLM2825014 119722332 ppf: 1427 ppct: 6 formats: tig: atl: Latent herpes simplex virus in human trigeminal ganglia. Detection of an immediate early gene "anti-sense" transcript by in situ hybridization. aug: au: Croen, K D Ostrove, J M Dragovic, L J Smialek, J E Straus, S E affil: Medical Virology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Md sug: subj: Proteins Genes RNA Herpes Simplex Microbiology Herpesviruses Trigeminal Nerve Microbiology Ganglia, Sensory Microbiology Middle Age Herpesviruses Immunology Adult Enzymes Aged Child Nucleic Acid Hybridization Adolescence RNA Analysis Antibodies, Viral Analysis Human Middle Aged: 45-64 years Adult: 19-44 years Aged: 65+ years Child: 6-12 years Adolescent: 13-18 years ab: We used in situ hybridization to study the expression of herpes simplex virus type 1 genes during latent infections of human sensory ganglia. Trigeminal ganglia were recovered at autopsy from 24 subjects with no evidence of an active herpetic infection. These ganglia were hybridized to 35S-labeled single-stranded RNA probes spanning 72 percent of the herpes simplex genome. In the ganglia of 16 subjects, 0.2 to 4.3 percent of the neuronal cells contained abundant nuclear signals for viral RNA. Ganglia from three patients with low or undetectable levels of antibodies to herpes simplex type 1 lacked viral RNA signals, whereas the ganglia from all of six patients with elevated antibody titers showed viral RNA signals. Transcription was detected only from the region of the viral genome containing a gene that encodes an immediate early protein known as the infected-cell protein number zero (ICP0). However, normal ("sense") transcripts of this gene, which are prominent in an acute infection, were not detected. In contrast, a novel transcript was found overlapping with, but opposite in direction to, the ICP0 transcript (and was therefore "anti-sense"). Although this transcript has been only partially characterized, we believe that it may have a role in maintaining the latency of herpes simplex virus. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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