Analysis of Gene Expression in Postmortem Supravital Time.
Objective: The objective of this study was to analyze the postmortem gene expression by time-dependent observation. Design: The post-mortem spleen samples were taken from the mice sequentially and RNA analysis were performed for time-dependent observation. Materials and methods: Mice were killed by...
| Publicado en: | International Medical Journal Vol. 23; no. 6; pp. 670 - 674 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Japan International Cultural Exchange Foundation
Dec2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=120656014&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 120656014 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13412051 23J1 jtl: International Medical Journal issn: 13412051 maglogo: N pubinfo: dt: Dec2016 vid: 23 iid: 6 pid: 29386 pub: Japan International Cultural Exchange Foundation artinfo: ui: 120656014 120656014 120656014 120656014 ppf: 670 ppct: 4 formats: fmt: @attributes: type: P tig: atl: Analysis of Gene Expression in Postmortem Supravital Time. aug: au: Toshiko Sawaguchi Yuuichirou Ishikawa Akiko Sawaguchi affil: Planning Div. INFRONT Inc sug: subj: Gene Expression Postmortem Care Spleen In Vitro Studies Animal Studies Mice Reverse Transcriptase Polymerase Chain Reaction Electrophoresis Blotting, Northern Oligonucleotide Array Sequence Analysis Microarray Analysis Funding Source ab: Objective: The objective of this study was to analyze the postmortem gene expression by time-dependent observation. Design: The post-mortem spleen samples were taken from the mice sequentially and RNA analysis were performed for time-dependent observation. Materials and methods: Mice were killed by cervical spine subluxation with anesthetic treatment. Total RNA was extracted from incident spleen from mice and clarified the quality and quantity. To clarify the postmortem change, the differential display-PCR were performed and followed by cloning the different amplifications, DNA sequencing and homology searches, RI labelling the cloned fragments and northern blotting. Results: Postmortem RNA stability at postmortem 60h has decreased than at postmortem(pm) Oh. One clone of differential display(DD)-polymerase chain reaction(PCR) products more increased even at pm 60h than at pm Oh. The cDNA clones from this cloned DD-PCR product indicated 100% homology toward the cDNA clones in Gen Bank (Mus musculus LOC229510, mRNA). Conclusion: Even in postmortem time, increased gene expression could be observed. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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