Cellular Senescence Biomarker p16INK4a+ Cell Burden in Thigh Adipose is Associated With Poor Physical Function in Older Women.
Background: Ample evidence implicates cellular senescence as a contributor to frailty and functional decline in rodents, but considerable effort remains to translate these findings to human aging.Methods: We quantified senescence biomarker p16INK4a-expressing cells in thigh adipose tissue obtained f...
| Publicado en: | Journals of Gerontology Series A: Biological Sciences & Medical Sciences Vol. 73; no. 7; pp. 939 - 946 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
Jul2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=130236033&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 130236033 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10795006 JGA jtl: Journals of Gerontology Series A: Biological Sciences & Medical Sciences issn: 10795006 maglogo: N pubinfo: dt: Jul2018 vid: 73 iid: 7 pid: 622 pub: Oxford University Press / USA artinfo: ui: 130236033 130236033 NLM28658942 130236033 10.1093/gerona/glx134 NLM28658942 130236033 ppf: 939 ppct: 7 formats: tig: atl: Cellular Senescence Biomarker p16INK4a+ Cell Burden in Thigh Adipose is Associated With Poor Physical Function in Older Women. aug: au: Justice, Jamie N. Gregory, Heather Tchkonia, Tamar LeBrasseur, Nathan K. Kirkland, James L. Kritchevsky, Stephen B. Nicklas, Barbara J. affil: Sticht Center for Healthy Aging and Alzheimer's Prevention, Internal Medicine - Gerontology and Geriatric Medicine, Wake Forest School of Medicine (WFSM), Winston-Salem, North Carolina sug: subj: Adipose Tissue Metabolism Aging Aging Physiology Proteins Metabolism Adipose Tissue Physiopathology Obesity Pathology Resistance Training Obesity Physiopathology Human Frail Elderly Adipose Tissue Pathology Adipose Tissue Distribution Female Obesity Metabolism Cross Sectional Studies Body Mass Index Thigh Diet Therapy Aged Validation Studies Comparative Studies Evaluation Research Multicenter Studies Clinical Assessment Tools Scales Funding Source Aged: 65+ years Female ab: Background: Ample evidence implicates cellular senescence as a contributor to frailty and functional decline in rodents, but considerable effort remains to translate these findings to human aging.Methods: We quantified senescence biomarker p16INK4a-expressing cells in thigh adipose tissue obtained from older women previously enrolled in a 5-month resistance training intervention, with or without caloric restriction (RT ± CR, n = 11 baseline, 8 pre-post-intervention pairs). Women in this subsample were older (72.9 ± 3.4 y) and overweight/obese (body mass index: 30.6 ± 2.4 kg/m2). p16INK4a+ cells were identified from 12 to 20 random visual fields/sample at 20× magnification (immunohistochemical, nuclear staining) and were present in all adipose samples.Results: Cross-sectional associations were observed between p16INK4a+ cell burden and physical function, including grip strength (r = -0.74), 400-m walk time (r = 0.74), 4-m gait speed (r = -0.73), and self-perceived mobility (r = -0.78) (p ≤ .05). These relationships remained significant after independent adjustments for age and adiposity (p ≤ .05). p16INK4a+ cell abundance was lower following the intervention (pre: 5.47 ± 3.4%, post: 2.17 ± 1.1% count p16INK4a+ cells, p ≤ .05).Conclusions: These results provide proof-of-concept that p16INK4a+ cells in thigh adipose are associated with physical function, and may be sensitive to change with RT ± CR in overweight/obese older women. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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